Adipose-derived regenerative cells for treating liver injury

• US 9,198,937 B2
• Filed: 12/21/2012
• Issued: 12/01/2015
• Est. Priority Date: 12/07/2001
• Status: Expired due to Term

• Current Assignee: Lorem Vascular Pte. Ltd.
• Original Assignee: Cytori Therapeutics Incorporated
• Inventors: Fraser, John K., Hedrick, Marc H.
• Primary Examiner(s): Lankford, Blaine
• Application Number:
  US13/725,814
• Publication Number:
  US 20130121974A1
• Time in Patent Office: 1,075 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A61B 10/0283 with vacuum aspiration, e.g. caused by retractable plunger or by connected syringe A61B10/025, A61B10/0266 take precedence
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 35/35 Fat tissue; Adipocytes; Stromal cells; Connective tissues adipose-derived stem cells A61K35/28; collagen A61K38/39
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61M 1/76 Handpieces specially for suction-irrigation A61M1/774, aspiration tips A61M1/84
• CPC Class Codes: A61M 2202/08 Lipoids
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 13/12 of the kidneys
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 19/04 for non-specific disorders of the connective tissue
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: A61P 19/10 for osteoporosis
• CPC Class Codes: A61P 21/00 Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 27/02 : Ophthalmic agents
• CPC Class Codes: A61P 3/00 : Drugs for disorders of the metabolism of the blood or the extracellular fluid A61P7/00
• CPC Class Codes: A61P 3/06 : Antihyperlipidemics
• CPC Class Codes: A61P 3/10 : for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 37/06 : Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 : Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/10 : for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: C12M 47/04 : Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: C12N 2506/1384 : from adipose-derived stem cells [ADSC], from adipose stromal stem cells
• CPC Class Codes: C12N 5/0667 : Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
• CPC Class Codes: C12N 5/069 : Vascular Endothelial cells

Abstract

Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient.

Claims

1. A method of treating alcohol-induced liver injury in a patient in need thereof, comprising:

(a) identifying a patient with alcohol-induced liver injury;

(b) providing a tissue removal system;

(c) removing adipose tissue from a patient using the tissue removal system;

(d) processing at least part of the adipose tissue to obtain a concentrated population of adipose-derived cells comprising stem cells, wherein the concentration of stem cells is at least 0.1% of the adipose-derived cell population, wherein the processing comprises

disaggregating the adipose tissue to free adipocytes and other cell populations from adipose tissue matrix to obtain a cell suspension;

reducing the presence of free adipocytes in the cell suspension; and

concentrating the cell suspension, to obtain the concentrated population of adipose-derived cells comprising stem cells; and

(e) administering the concentrated population of adipose-derived cells comprising stem cells to the patient with alcohol-induced liver injury.

2. The method of claim 1, wherein the liver injury is alcohol-induced liver injury.

3. The method of claim 1, wherein the concentrated population of adipose-derived cells comprising stem cells is administered to the patient intravenously.

4. The method of claim 1, wherein the concentrated population of adipose-derived cells comprising stem cells is administered to the patient through the hepatic circulatory system.

5. The method of claim 1, wherein the concentrated population of adipose-derived cells comprising stem cells is cultured prior to administration to the patient.

6. The method of claim 1, wherein the concentrated population of adipose-derived cells comprising stem cells is not cultured prior to administration to the patient.

7. The method of claim 1, wherein the tissue removal system comprises:

a tissue collecting container configured to receive and fully or partially disaggregate adipose tissue, wherein said tissue collecting container is coupled to a cell collection container wherein said coupling defines a closed system in which said fully or partially disaggregated adipose tissue directed from tissue collecting container to cell collection container is not exposed to the external environment; and

wherein said cell collection container, further comprises a cell concentrator that facilitates separation of the cells in a suspension; and

an outlet that is structured to permit said concentrated population of cells comprising adipose-derived cells to be removed from said adipose tissue processing unit.

8. The method of claim 1, wherein the therapeutically effective amount of the concentrated population of adipose-derived regenerative cells comprises a minimum concentration of between 1×

10⁵ and 1×

10⁷ adipose-derived stem cells.

9. The method of claim 1, wherein the concentrated population of cells comprising adipose-derived stem cells is processed to remove unwanted cell and tissue aggregates prior to administration.

10. The method of claim 1, wherein the concentrated population of cells comprising adipose-derived stem cells comprises an additive.

11. The method of claim 10, wherein the additive is selected from the group consisting of other cells, tissue, and tissue fragments.

12. The method of claim 10, wherein the additive is selected from the group consisting of cytokines, growth factors and immunosuppressive agents.

13. The method of claim 1, wherein the concentrated population of adipose-derived cells comprising stem cells is administered to the patient.

14. The method of claim 1, wherein the stem cells comprise more than 0.1% of the cellular component of the concentrated population of adipose-derived cells.