Hybrid soft tissue implants from progenitor cells and biomaterials
First Claim
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1. A method of forming a hybrid soft tissue construct, the hybrid soft tissue construct comprising a core material, a biomaterial matrix, and isolated mammalian cells, the method comprising:
- providing the isolated mammalian cells, the biomaterial matrix and the core material;
contacting the mammalian cells and the biomaterial matrix;
contacting the biomaterial matrix and the core material;
forming a plurality of macrochannels in the biomaterial matrix, the plurality of macrochannels not exposing the core material underlying the biomaterial matrix; and
incubating the biomaterial matrix, the mammalian cells, and the core material to form the hybrid soft tissue construct;
wherein,the biomaterial matrix is a polyethylene glycol diacrylate (PEGDA) hydrogel further comprising the mammalian cells;
the hydrogel with the mammalian cells are contacted with the core material as a liquid then photopolymerized into a gel;
formation of the macrochannels comprises placing capillary tubes radially at locations near the core material before the core material and the hydrogel with the mammalian cells are contacted;
each of the capillary tubes is removed after the hydrogel with the mammalian cells contact the core material and after the hydrogel photopolymerizes into the gel;
the core material is acellular and biocompatible, and designed to remain acellular upon implantation in a subject;
the biomaterial matrix comprises a plurality of microchannel pores throughout the biomaterial matrix;
the plurality of macrochannels have an average diameter of 0.1 mm to 50 mm;
the plurality of macrochannels does not expose the core material to host tissue; and
the biomaterial matrix contacts and covers the acellular core.
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Abstract
Provided are hybrid soft tissue constructs comprising a core material, a biomaterial matrix and mammalian cells. Also provided are methods of augmenting or reconstructing a soft tissue of a mammal. Additionally, methods of forming a hybrid soft tissue construct are provided. The use of the above constructs for augmenting or reconstructing a soft tissue of a mammal are further provided. Additionally provided is the use of the above constructs for the manufacture of a medicament for augmenting or reconstructing a soft tissue of a mammal.
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Citations
17 Claims
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1. A method of forming a hybrid soft tissue construct, the hybrid soft tissue construct comprising a core material, a biomaterial matrix, and isolated mammalian cells, the method comprising:
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providing the isolated mammalian cells, the biomaterial matrix and the core material; contacting the mammalian cells and the biomaterial matrix; contacting the biomaterial matrix and the core material; forming a plurality of macrochannels in the biomaterial matrix, the plurality of macrochannels not exposing the core material underlying the biomaterial matrix; and incubating the biomaterial matrix, the mammalian cells, and the core material to form the hybrid soft tissue construct; wherein, the biomaterial matrix is a polyethylene glycol diacrylate (PEGDA) hydrogel further comprising the mammalian cells; the hydrogel with the mammalian cells are contacted with the core material as a liquid then photopolymerized into a gel; formation of the macrochannels comprises placing capillary tubes radially at locations near the core material before the core material and the hydrogel with the mammalian cells are contacted; each of the capillary tubes is removed after the hydrogel with the mammalian cells contact the core material and after the hydrogel photopolymerizes into the gel; the core material is acellular and biocompatible, and designed to remain acellular upon implantation in a subject; the biomaterial matrix comprises a plurality of microchannel pores throughout the biomaterial matrix; the plurality of macrochannels have an average diameter of 0.1 mm to 50 mm; the plurality of macrochannels does not expose the core material to host tissue; and the biomaterial matrix contacts and covers the acellular core. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification