Flow control device for assays
First Claim
1. A fluidic device for flow control in an assay comprising:
- a water impermeable substrate with a flow channel located on its upper surface;
a porous reagent pad located within the flow channel, where the reagent pad includes a release zone that comprises a mobilizable reagent component of an assay;
a porous sensor membrane located within the flow channel downstream from the reagent pad, where the sensor membrane is separated from the reagent pad by a free space diffusion zone and where the sensor membrane includes a capture zone that comprises an immobilized capture component of the assay;
a water impermeable top support located within the flow channel and disposed over at least a portion of the sensor membrane; and
a flow control medium that forms a water impermeable seal around a portion of the top support and sensor membrane, where the seal is configured to direct flow of fluid into the sealed portion of the sensor membrane.
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Accused Products
Abstract
The present disclosure relates to devices and methods for detecting the presence of a target analyte in a fluid sample using an assay. A fluidic device for flow control in an assay is disclosed comprising a water impermeable substrate (300) with a flow channel (301) located on its upper surface; a porous reagent pad (305) located within the flow channel, where the reagent pad includes a release zone that comprises a mobilizable reagent component of an assay; a porous sensor membrane (306) located within the flow channel downstream from the reagent pad, where the sensor membrane is separated from the reagent pad by a free space diffusion zone and where the sensor membrane includes a capture zone that comprises an immobilized capture component of the assay; a water impermeable top support located within the flow channel and disposed over at least a portion of the sensor membrane; and a flow control medium that forms a water impermeable seal around a portion of the top support and sensor membrane, where the seal is configure to direct flow of fluid into the sealed portion of the sensor membrane.
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Citations
20 Claims
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1. A fluidic device for flow control in an assay comprising:
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a water impermeable substrate with a flow channel located on its upper surface; a porous reagent pad located within the flow channel, where the reagent pad includes a release zone that comprises a mobilizable reagent component of an assay; a porous sensor membrane located within the flow channel downstream from the reagent pad, where the sensor membrane is separated from the reagent pad by a free space diffusion zone and where the sensor membrane includes a capture zone that comprises an immobilized capture component of the assay; a water impermeable top support located within the flow channel and disposed over at least a portion of the sensor membrane; and a flow control medium that forms a water impermeable seal around a portion of the top support and sensor membrane, where the seal is configured to direct flow of fluid into the sealed portion of the sensor membrane. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19, 20)
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18. A method of making a fluidic device for flow control in an assay comprising steps of:
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providing a water impermeable substrate with a flow channel located on its upper surface; placing a porous reagent pad within the flow channel, where the reagent pad includes a release zone that comprises a mobilizable reagent component of an assay; placing a porous sensor membrane within the flow channel downstream from the reagent pad, where the sensor membrane is separated from the reagent pad by a free space diffusion zone and where the sensor membrane includes a capture zone that comprises an immobilized capture component of the assay; placing a water impermeable top support within the flow channel and over at least a portion of the sensor membrane; and introducing a flow control medium that forms a water impermeable seal around a portion of the top support and sensor membrane, where the seal is configured to direct flow of fluid from the free space diffusion zone into the sealed portion of the sensor membrane.
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Specification