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Flow control device for assays

  • US 9,199,232 B2
  • Filed: 04/06/2011
  • Issued: 12/01/2015
  • Est. Priority Date: 04/07/2010
  • Status: Active Grant
First Claim
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1. A fluidic device for flow control in an assay comprising:

  • a water impermeable substrate with a flow channel located on its upper surface;

    a porous reagent pad located within the flow channel, where the reagent pad includes a release zone that comprises a mobilizable reagent component of an assay;

    a porous sensor membrane located within the flow channel downstream from the reagent pad, where the sensor membrane is separated from the reagent pad by a free space diffusion zone and where the sensor membrane includes a capture zone that comprises an immobilized capture component of the assay;

    a water impermeable top support located within the flow channel and disposed over at least a portion of the sensor membrane; and

    a flow control medium that forms a water impermeable seal around a portion of the top support and sensor membrane, where the seal is configured to direct flow of fluid into the sealed portion of the sensor membrane.

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