Multiwell plate
First Claim
1. A multiwell plate comprising a unitary structure including a plurality of wells wherein the bottom surface of at least one well of said plurality of wells includes a plurality of picowells, wherein said picowells are unitary structures each comprised of a bottom and a side wall integrally formed with said bottom, and the bottoms and side walls of said picowells include a coating of a material that inhibits or delays adherence of cells in said picowells.
1 Assignment
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Accused Products
Abstract
A mulitwell plate having a plurality of picowells on the bottom of the wells of the plate as well as methods of producing the mulitwell plate are provided. Provided is also a method of handling living cells by providing an ordered array of living cells immobilized in a non-fluid matrix, contacting the living cells with a stimulus; and detecting a response to the stimulus. The present invention is also of a method of producing an ordered array of living cells.
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Citations
58 Claims
- 1. A multiwell plate comprising a unitary structure including a plurality of wells wherein the bottom surface of at least one well of said plurality of wells includes a plurality of picowells, wherein said picowells are unitary structures each comprised of a bottom and a side wall integrally formed with said bottom, and the bottoms and side walls of said picowells include a coating of a material that inhibits or delays adherence of cells in said picowells.
- 22. A multiwell plate comprising a unitary structure including a plurality of wells wherein at the bottom surface of at least one well of said plurality of wells is includes a plurality of picowells, wherein said picowells are unitary structures each comprised of a bottom and a side wall integrally formed with said bottom, and wherein the bottoms and side walls of said picowells include a coating of a material that affects proliferation of cells in said picowells.
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43. A method of making a multiwell plate comprised of an integral structure formed of a plurality of wells and a plurality of picowells on the bottom of at least one of said wells, the method comprising:
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(a) placing a precursor material on a substrate; (b) contacting said precursor material with a template including a negative of one or more features of said plate including said picowells; (b) fixing said one or more features in said precursor material so as to fashion an incipient plate; and (c) processing said incipient plate so as to fashion said unitary structure of said plurality of wells and said plurality of picowells with said picowells attached to a bottom surface of at least one of said wells as part of said unitary structure; and (d) coating the bottoms and side walls of said picowells with a material that affects proliferation of cells in said picowells. - View Dependent Claims (44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58)
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Specification