Thermal cycling reaction block and continuous real-time monitoring apparatus using the same
First Claim
1. A real-time monitoring apparatus comprising:
- a thermal cycling reaction block 100, comprisinga doughnut-shaped heating block 10, said doughnut-shaped heating block comprising a region 10a, a region 10b, and an insulating layer 30 which is placed between the region 10a and the region 10b so as to separate the region 10a from the region 10b, wherein said region 10a, insulating layer 30, and region 10b are circular around a common axis at the center of the doughnut-shaped heating block 10 and wherein a temperature of the region 10a is different from a temperature of the region 10b; and
a capillary tube 20 wound around the doughnut-shaped heating block 10 through a hollow part 11 of the doughnut-shaped heating block 10 at a regular interval to as to a respective individual turn of the wounded capillary tube 20 be in contact with surfaces of the region 10a, insulation layer 30, region 10b, insulation layer 30, and region 10a in this order, wherein the capillary tube 20 has an inlet to receive a sample to be subjected to a thermal cycling reaction and an outlet to output the sample after the thermal cycling reaction;
a light source 110 for irradiating excitation light;
a band pass filter 130 for passing the excitation light having only a desired wavelength irradiated from the light source 110;
a first condensing lens 140 for condensing the excitation light;
a beam splitter 120 which reflects the excitation light and passes fluorescence generated from a sample in a capillary tube 20;
a reflecting mirror 150 which is rotatably connected with a motor 160 so as to transfer the excitation light reflected from the beam splitter 120 to the capillary tube 20 and reflect the fluorescence generated from the sample in the capillary tube 20, wherein the reflecting mirror 150 is disposed at the hollow part 11 formed at the central portion of the thermal cycling reaction block 100; and
a fluorescence detecting part 170 for detecting the fluorescence that is reflected by the reflecting mirror 150 and then passes through the beam splitter 120.
1 Assignment
0 Petitions
Accused Products
Abstract
Disclosed is real-time monitoring apparatus comprising a thermal cycling reaction block having heating block which is formed of a hollow part and divided by an insulating layer, and a capillary tube through which a sample is flowed in and/or out and which is wound on the heating block so that the different temperatures are transferred and thus reaction cycle is repeatedly performed; a light source; a band pass filter; a condensing lens; a beam splitter; a reflecting mirror which is rotatably connected with a motor so as to transfer the excitation light reflected from the beam splitter to the capillary tube and reflect the fluorescence generated from the sample in the capillary tube; and a fluorescence detecting part.
10 Citations
25 Claims
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1. A real-time monitoring apparatus comprising:
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a thermal cycling reaction block 100, comprising a doughnut-shaped heating block 10, said doughnut-shaped heating block comprising a region 10a, a region 10b, and an insulating layer 30 which is placed between the region 10a and the region 10b so as to separate the region 10a from the region 10b, wherein said region 10a, insulating layer 30, and region 10b are circular around a common axis at the center of the doughnut-shaped heating block 10 and wherein a temperature of the region 10a is different from a temperature of the region 10b; and a capillary tube 20 wound around the doughnut-shaped heating block 10 through a hollow part 11 of the doughnut-shaped heating block 10 at a regular interval to as to a respective individual turn of the wounded capillary tube 20 be in contact with surfaces of the region 10a, insulation layer 30, region 10b, insulation layer 30, and region 10a in this order, wherein the capillary tube 20 has an inlet to receive a sample to be subjected to a thermal cycling reaction and an outlet to output the sample after the thermal cycling reaction; a light source 110 for irradiating excitation light; a band pass filter 130 for passing the excitation light having only a desired wavelength irradiated from the light source 110; a first condensing lens 140 for condensing the excitation light; a beam splitter 120 which reflects the excitation light and passes fluorescence generated from a sample in a capillary tube 20; a reflecting mirror 150 which is rotatably connected with a motor 160 so as to transfer the excitation light reflected from the beam splitter 120 to the capillary tube 20 and reflect the fluorescence generated from the sample in the capillary tube 20, wherein the reflecting mirror 150 is disposed at the hollow part 11 formed at the central portion of the thermal cycling reaction block 100; and a fluorescence detecting part 170 for detecting the fluorescence that is reflected by the reflecting mirror 150 and then passes through the beam splitter 120. - View Dependent Claims (2, 3, 4)
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5. A real-time monitoring apparatus comprising:
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a thermal cycling reaction block 100, comprising a doughnut-shaped first heating block 10, said doughnut-shaped heating block comprising a region 10a, a region 10b, and an insulating layer 30 which is placed between the region 10a and the region 10b so as to separate the region 10a from the region 10b, wherein said region 10a, insulating layer 30, and region 10b are circular around a common axis at the center of the doughnut-shaped heating block 10 and wherein a temperature of the region 10a is different from a temperature of the region 10b;
a capillary tube 20 wound around the doughnut-shaped heating block 10 through a hollow part 11 of the doughnut-shaped heating block 10 at a regular interval to as to a respective individual turn of the wounded capillary tube 20 be in contact with surfaces of the region 10a, insulation layer 30, region 10b, insulation layer 30, and region 10a in this order, wherein the capillary tube 20 has an inlet to receive a sample to be subjected to a thermal cycling reaction and an outlet to output the sample after the thermal cycling reaction; and a second heating block 13 located in contact with outer side of the doughnut-shaped first heating block 10, a light source 110 for irradiating excitation light; a band pass filter 130 for passing the excitation light having only a desired wavelength irradiated from the light source 110; a first condensing lens 140 for condensing the excitation light; a beam splitter 120 which reflects the excitation light and passes fluorescence generated from a sample in a capillary tube 20; a reflecting mirror 150 which is rotatably connected with a motor 160 so as to transfer the excitation light reflected from the beam splitter 120 to the capillary tube 20 and reflect the fluorescence generated from the sample in the capillary tube 20, wherein the reflecting mirror 150 is disposed at the hollow part 11 formed at the central portion of the thermal cycling reaction block 100; and a fluorescence detecting part 170 for detecting the fluorescence that is reflected by the reflecting mirror 150 and then passes through the beam splitter 120. - View Dependent Claims (6, 7, 8)
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9. A real-time monitoring apparatus comprising:
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a thermal cycling reaction block 100, a doughnut-shaped heating block 10, said doughnut-shaped heating block comprising a region 10a, a region 10b, and an insulating layer 30 which is placed between the region 10a and the region 10b so as to separate the region 10a from the region 10b, wherein said region 10a, insulating layer 30, and region 10b are circular around a common axis at the center of the doughnut-shaped heating block 10 and wherein a temperature of the region 10a is different from a temperature of the region 10b; and a capillary tube 20 wound around the doughnut-shaped heating block 10 through a hollow part 11 of the doughnut-shaped heating block 10 at a regular interval to as to a respective individual turn of the wounded capillary tube 20 be in contact with surfaces of the region 10a, insulation layer 30, region 10b, insulation layer 30, and region 10a in this order, wherein the capillary tube 20 has an inlet to receive a sample to be subjected to a thermal cycling reaction and an outlet to output the sample after the thermal cycling reaction, wherein the doughnut-shaped heating block 10 is provided with a groove 12 on outer surface of the block 10 into which the capillary tube 20 is inserted; a light source 110 for irradiating excitation light; a band pass filter 130 for passing the excitation light having only a desired wavelength irradiated from the light source 110; a first condensing lens 140 for condensing the excitation light; a beam splitter 120 which reflects the excitation light and passes fluorescence generated from a sample in a capillary tube 20; a reflecting mirror 150 which is rotatably connected with a motor 160 so as to transfer the excitation light reflected from the beam splitter 120 to the capillary tube 20 and reflect the fluorescence generated from the sample in the capillary tube 20, wherein the reflecting mirror 150 is disposed at the hollow part 11 formed at the central portion of the thermal cycling reaction block 100; and a fluorescence detecting part 170 for detecting the fluorescence that is reflected by the reflecting mirror 150 and then passes through the beam splitter 120. - View Dependent Claims (10, 11, 12, 13)
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14. A real-time monitoring apparatus comprising:
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a thermal cycling reaction block, comprising a doughnut-shaped first heating block 10, said doughnut-shaped heating block comprising a region 10a, a region 10b, and an insulating layer 30 which is placed between the region 10a and the region 10b so as to separate the region 10a from the region 10b, wherein said region 10a, insulating layer 30, and region 10b are circular around a common axis at the center of the doughnut-shaped heating block 10 and wherein a temperature of the region 10a is different from a temperature of the region 10b;
a capillary tube 20 wound around the doughnut-shaped heating block 10 through a hollow part 11 of the doughnut-shaped heating block 10 at a regular interval to as to a respective individual turn of the wounded capillary tube 20 be in contact with surfaces of the region 10a, insulation layer 30, region 10b, insulation layer 30, and region 10a in this order, wherein the capillary tube 20 has an inlet to receive a sample to be subjected to a thermal cycling reaction and an outlet to output the sample after the thermal cycling reaction; a light source 110 for irradiating excitation light; a band pass filter 130 for passing only the excitation light having a desired wavelength irradiated from the light source 110; a first condensing lens 140 for condensing the excitation light; a beam splitter 120 which reflects the excitation light and passes fluorescence generated from a sample in a capillary tube 20; a reflecting mirror 150 which is rotatably connected with a first motor 160a so as to transfer the excitation light reflected from the beam splitter 120 to the capillary tube 20 and reflect the fluorescence generated from the sample in the capillary tube 20, wherein the reflecting mirror 150 is disposed at the hollow part 11 formed at the central portion of the thermal cycling reaction block 100; a second condensing lens 141 which is positioned between the reflecting minor 150 and the thermal cycling reaction block 100 so as to condense the excitation light reflected from the reflecting mirror 150 and the fluorescence generated from a sample in a capillary tube 20; and a fluorescence detecting part 170 for detecting the fluorescence that is reflected by the reflecting mirror 150 and then passes through the beam splitter 120. - View Dependent Claims (15, 16, 17)
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18. A real-time monitoring apparatus comprising:
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a thermal cycling reaction block, comprising a doughnut-shaped first heating block 10, said doughnut-shaped heating block comprising a region 10a, a region 10b, and an insulating layer 30 which is placed between the region 10a and the region 10b so as to separate the region 10a from the region 10b, wherein said region 10a, insulating layer 30, and region 10b are circular around a common axis at the center of the doughnut-shaped heating block 10 and wherein a temperature of the region 10a is different from a temperature of the region 10b;
a capillary tube 20 wound around the doughnut-shaped heating block 10 through a hollow part 11 of the doughnut-shaped heating block 10 at a regular interval to as to a respective individual turn of the wounded capillary tube 20 be in contact with surfaces of the region 10a, insulation layer 30, region 10b, insulation layer 30, and region 10a in this order, wherein the capillary tube 20 has an inlet to receive a sample to be subjected to a thermal cycling reaction and an outlet to output the sample after the thermal cycling reaction; and a second heating block 13 located in contact with outer side of the doughnut-shaped first heating block 10, a light source 110 for irradiating excitation light; a band pass filter 130 for passing only the excitation light having a desired wavelength irradiated from the light source 110; a first condensing lens 140 for condensing the excitation light; a beam splitter 120 which reflects the excitation light and passes fluorescence generated from a sample in a capillary tube 20; a reflecting mirror 150 which is rotatably connected with a first motor 160a so as to transfer the excitation light reflected from the beam splitter 120 to the capillary tube 20 and reflect the fluorescence generated from the sample in the capillary tube 20; a second condensing lens 141 which is positioned between the reflecting minor 150 and the thermal cycling reaction block 100 so as to condense the excitation light reflected from the reflecting mirror 150 and the fluorescence generated from a sample in a capillary tube 20, wherein the reflecting minor 150 is disposed at the hollow part 11 formed at the central portion of the thermal cycling reaction block 100; and a fluorescence detecting part 170 for detecting the fluorescence that is reflected by the reflecting mirror 150 and then passes through the beam splitter 120. - View Dependent Claims (19, 20, 21)
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22. A real-time monitoring apparatus comprising:
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a thermal cycling reaction block, comprising a doughnut-shaped heating block 10, said doughnut-shaped heating block comprising a region 10a, a region 10b, and an insulating layer 30 which is placed between the region 10a and the region 10b so as to separate the region 10a from the region 10b, wherein said region 10a, insulating layer 30, and region 10b are circular around a common axis at the center of the doughnut-shaped heating block 10 and wherein a temperature of the region 10a is different from a temperature of the region 10b; and a capillary tube 20 wound around the doughnut-shaped heating block 10 through a hollow part 11 of the doughnut-shaped heating block 10 at a regular interval to as to a respective individual turn of the wounded capillary tube 20 be in contact with surfaces of the region 10a, insulation layer 30, region 10b, insulation layer 30, and region 10a in this order, wherein the capillary tube 20 has an inlet to receive a sample to be subjected to a thermal cycling reaction and an outlet to output the sample after the thermal cycling reaction, wherein the doughnut-shaped heating block 10 is provided with a groove 12 on outer surface of the block 10 into which the capillary tube 20 is inserted; a light source 110 for irradiating excitation light; a band pass filter 130 for passing only the excitation light having a desired wavelength irradiated from the light source 110; a first condensing lens 140 for condensing the excitation light; a beam splitter 120 which reflects the excitation light and passes fluorescence generated from a sample in a capillary tube 20; a reflecting mirror 150 which is rotatably connected with a first motor 160a so as to transfer the excitation light reflected from the beam splitter 120 to the capillary tube 20 and reflect the fluorescence generated from the sample in the capillary tube 20; a second condensing lens 141 which is positioned between the reflecting minor 150 and the thermal cycling reaction block 100 so as to condense the excitation light reflected from the reflecting mirror 150 and the fluorescence generated from a sample in a capillary tube 20, wherein the reflecting minor 150 is disposed at the hollow part 11 formed at the central portion of the thermal cycling reaction block 100; and a fluorescence detecting part 170 for detecting the fluorescence that is reflected by the reflecting mirror 150 and then passes through the beam splitter 120. - View Dependent Claims (23, 24, 25)
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Specification