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Thermal cycling reaction block and continuous real-time monitoring apparatus using the same

  • US 9,205,425 B2
  • Filed: 06/23/2009
  • Issued: 12/08/2015
  • Est. Priority Date: 06/23/2008
  • Status: Active Grant
First Claim
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1. A real-time monitoring apparatus comprising:

  • a thermal cycling reaction block 100, comprisinga doughnut-shaped heating block 10, said doughnut-shaped heating block comprising a region 10a, a region 10b, and an insulating layer 30 which is placed between the region 10a and the region 10b so as to separate the region 10a from the region 10b, wherein said region 10a, insulating layer 30, and region 10b are circular around a common axis at the center of the doughnut-shaped heating block 10 and wherein a temperature of the region 10a is different from a temperature of the region 10b; and

    a capillary tube 20 wound around the doughnut-shaped heating block 10 through a hollow part 11 of the doughnut-shaped heating block 10 at a regular interval to as to a respective individual turn of the wounded capillary tube 20 be in contact with surfaces of the region 10a, insulation layer 30, region 10b, insulation layer 30, and region 10a in this order, wherein the capillary tube 20 has an inlet to receive a sample to be subjected to a thermal cycling reaction and an outlet to output the sample after the thermal cycling reaction;

    a light source 110 for irradiating excitation light;

    a band pass filter 130 for passing the excitation light having only a desired wavelength irradiated from the light source 110;

    a first condensing lens 140 for condensing the excitation light;

    a beam splitter 120 which reflects the excitation light and passes fluorescence generated from a sample in a capillary tube 20;

    a reflecting mirror 150 which is rotatably connected with a motor 160 so as to transfer the excitation light reflected from the beam splitter 120 to the capillary tube 20 and reflect the fluorescence generated from the sample in the capillary tube 20, wherein the reflecting mirror 150 is disposed at the hollow part 11 formed at the central portion of the thermal cycling reaction block 100; and

    a fluorescence detecting part 170 for detecting the fluorescence that is reflected by the reflecting mirror 150 and then passes through the beam splitter 120.

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