Method and apparatus for processing adipose tissue
First Claim
1. An apparatus for collection of human biological material and post-collection processing of collected material, the apparatus being orientable in an access orientation, as oriented in the access orientation the apparatus comprising:
- a filter;
a container having an internal containment volume having a volume in a range of from 100 cubic centimeters to 1300 cubic centimeters, the internal containment volume comprising;
a tissue retention volume and a filtrate volume separated by the filter;
a collection volume within the filtrate volume, the collection volume having a bottom elevation corresponding to a bottom elevation of the filtrate volume and a top elevation that is lower than the bottom elevation of the tissue retention volume;
a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located below the bottom elevation of the tissue retention volume;
an inlet port in fluid communication with the tissue retention volume and configured for introducing human biological material directly into the tissue retention volume;
a suction port in fluid communication with the filtrate volume and providing access to the filtrate volume for suctioning from the filtrate volume components passing through the filter from the tissue retention volume to the filtrate volume; and
an extraction port in fluid communication with the internal containment volume and configured for removing processed biological material from the internal containment volume;
wherein each of the inlet port, the suction port and the extraction port is configured for access therethrough from above the container into the internal containment volume;
a mixing device disposed at least in part within the tissue retention volume for mixing contents within the tissue retention volume, wherein the mixing device comprises a rotatable shaft extending from outside of the internal containment volume to inside of the internal containment volume, wherein the shaft is rotatable about an axis that extends through the collection volume, the apparatus further comprising;
a lumen extending through the rotatable shaft and having a proximal end located outside of the internal containment volume and a distal end located within the internal containment volume, thereby providing access from outside of the internal containment volume to inside of the internal containment volume.
3 Assignments
0 Petitions
Accused Products
Abstract
A portable apparatus for collection and processing of human biological material, such as containing adipose extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. Inlet and suction ports provide access to the tissue retention volume and filtrate volume, respectively, and an extraction port provides versatile access for removal of target processed concentrate material or fat graft material, which access may be via a lumen through a rotatable mixer shaft. Access ports may be configured for access only from above the container. A method of processing adipose tissue to concentrate leuko stromal vascular cells includes multi-step processing using a portable container.
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Citations
33 Claims
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1. An apparatus for collection of human biological material and post-collection processing of collected material, the apparatus being orientable in an access orientation, as oriented in the access orientation the apparatus comprising:
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a filter; a container having an internal containment volume having a volume in a range of from 100 cubic centimeters to 1300 cubic centimeters, the internal containment volume comprising; a tissue retention volume and a filtrate volume separated by the filter; a collection volume within the filtrate volume, the collection volume having a bottom elevation corresponding to a bottom elevation of the filtrate volume and a top elevation that is lower than the bottom elevation of the tissue retention volume; a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located below the bottom elevation of the tissue retention volume; an inlet port in fluid communication with the tissue retention volume and configured for introducing human biological material directly into the tissue retention volume; a suction port in fluid communication with the filtrate volume and providing access to the filtrate volume for suctioning from the filtrate volume components passing through the filter from the tissue retention volume to the filtrate volume; and an extraction port in fluid communication with the internal containment volume and configured for removing processed biological material from the internal containment volume; wherein each of the inlet port, the suction port and the extraction port is configured for access therethrough from above the container into the internal containment volume; a mixing device disposed at least in part within the tissue retention volume for mixing contents within the tissue retention volume, wherein the mixing device comprises a rotatable shaft extending from outside of the internal containment volume to inside of the internal containment volume, wherein the shaft is rotatable about an axis that extends through the collection volume, the apparatus further comprising; a lumen extending through the rotatable shaft and having a proximal end located outside of the internal containment volume and a distal end located within the internal containment volume, thereby providing access from outside of the internal containment volume to inside of the internal containment volume. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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Specification