Systems and methods for detection and quantification of analytes
First Claim
1. An electrochemical sensor device for detection of a target analyte, comprising:
- at least one working electrode located within a microfluidic channel, wherein the working electrode is configured to release electrons to replenish electrons stripped from a substrate by at least one oxidizing enzyme in a quantity that correlates to a quantity of the target analyte when present in the microfluidic channel; and
at least one reference electrode located within the microfluidic channel,wherein a surface of the working electrode is chemically modified, andwherein the chemical modification of the surface comprises a thiolated ethylene glycol comprising a hexaethylene glycol dithiol.
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Accused Products
Abstract
Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.
96 Citations
16 Claims
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1. An electrochemical sensor device for detection of a target analyte, comprising:
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at least one working electrode located within a microfluidic channel, wherein the working electrode is configured to release electrons to replenish electrons stripped from a substrate by at least one oxidizing enzyme in a quantity that correlates to a quantity of the target analyte when present in the microfluidic channel; and at least one reference electrode located within the microfluidic channel, wherein a surface of the working electrode is chemically modified, and wherein the chemical modification of the surface comprises a thiolated ethylene glycol comprising a hexaethylene glycol dithiol. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification