Method and device for immunoassay using nucleotide conjugates
First Claim
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1. A single-use cartridge for performing an immunoassay, comprising:
- a sample entry port for receiving a blood sample suspected of containing a target analyte,a conduit containing an electrode,a region containing a wash fluid,a waste chamber,immobilized first antibody capable of binding to said target analyte,second antibody disposed in a solubilizing agent disposed within said conduit and capable of binding to said target analyte, wherein the second antibody is attached to a first single stranded nucleotide, andone or more signal elements each attached to a second single stranded nucleotide having complimentary base pairs to the first single stranded nucleotide;
wherein;
the wash fluid is capable of washing said blood sample from said electrode in said conduit into said waste chamber, anda 3′
-end or 5′
-end of the a first single stranded nucleotide and the a second single stranded nucleotide is protected from endogenous exonuclease activity from said blood sample within the conduit by incorporating a protective chemical group.
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Abstract
A composition of matter for use in an immunoassay devices and method comprising a signal antibody, e.g., FAB fragment, covalently linked to a first nucleotide; and one or more signal elements, e.g., signal enzymes such as ALP or fluorescent dyes, each covalently linked to a second nucleotide, wherein the first nucleotide has one or more repeated sequences, and the second nucleotide is bound to one of the one or more repeated sequences on said first nucleotide, and wherein the ratio of the signal antibody to the signal element is controlled by the number of repeated sequences.
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6 Claims
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1. A single-use cartridge for performing an immunoassay, comprising:
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a sample entry port for receiving a blood sample suspected of containing a target analyte, a conduit containing an electrode, a region containing a wash fluid, a waste chamber, immobilized first antibody capable of binding to said target analyte, second antibody disposed in a solubilizing agent disposed within said conduit and capable of binding to said target analyte, wherein the second antibody is attached to a first single stranded nucleotide, and one or more signal elements each attached to a second single stranded nucleotide having complimentary base pairs to the first single stranded nucleotide; wherein; the wash fluid is capable of washing said blood sample from said electrode in said conduit into said waste chamber, and a 3′
-end or 5′
-end of the a first single stranded nucleotide and the a second single stranded nucleotide is protected from endogenous exonuclease activity from said blood sample within the conduit by incorporating a protective chemical group. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification