Methods, devices, kits and compositions for detecting roundworm

• US 9,212,220 B2
• Filed: 08/14/2012
• Issued: 12/15/2015
• Est. Priority Date: 05/19/2008
• Status: Active Grant

• Current Assignee: Monsanto Company (Bayer AG), IDEXX Laboratories Incorporated
• Original Assignee: Divergence, Inc. (Bayer AG), IDEXX Laboratories Incorporated
• Inventors: Elsemore, David Allen, Flynn, Laurie A., Crawford, Michael, Geng, Jinming
• Primary Examiner(s): Baskar, Padma V
• Application Number:
  US13/585,429
• Publication Number:
  US 20130224759A1
• Time in Patent Office: 1,218 Days
• US Class Current: 1/1
• CPC Class Codes: A61K 39/395 Antibodies agglutinins A61K38/36 ; as drug carriers A61K47/50; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
• CPC Class Codes: A61K 39/44 Antibodies bound to carriers
• CPC Class Codes: C07K 14/4354 from nematodes
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Abstract

Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a mammalian sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

Claims

1. An isolated antibody wherein the antibody specifically binds to polypeptide Copro6716, a 10 kd isoform of a execretory/secretory protein of T. Canis obtained from a fecal sample of roundworm-infected mammal, wherein the antibody is immobilized on a solid support.

2. The isolated antibody of claim 1, wherein the antibody is obtained by immunization with the polypeptide Copro6716.

3. The isolated antibody of claim 1, wherein the antibody is detectably labeled.

4. An isolated antibody wherein the antibody specifically binds to polypeptide Copro6716, a 10 kd isoform of a execretory/secretory protein of T. Canis, wherein the antibody specifically binds roundworm antigen in a fecal sample obtained from a roundworm-infected mammal, wherein the antibody is immobilized on a solid support.

5. The isolated antibody of claim 4, wherein the mammal is further infected with one or more of hookworm, whipworm and heartworm and the antibody does not specifically bind to any antigen from the one or more of hookworm, whipworm, or heartworm that may be present in the fecal sample.