Sensor and concentration measurement method

• US 9,212,380 B2
• Filed: 08/30/2010
• Issued: 12/15/2015
• Est. Priority Date: 08/31/2009
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: Takahara, Yoshifumi, Nakaminami, Takahiro, Ikeda, Shin
• Primary Examiner(s): Ball, J. Christopher
• Application Number:
  US13/392,630
• Publication Number:
  US 20120152763A1
• Time in Patent Office: 1,933 Days
• Field of Search: 20440301-40315, 205/777.5, 205/792, 435/4, 435/7.6, 435/11, 435/14, 435 25- 28
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A reagent layer of a sensor contains as a mediator a quinone compound having a hydrophilic functional group, phenanthrenequinone, and/or a phenanthrenequinone derivative. The quinone compound has a lower redox potential than a conventional mediator, so interfering substances have less effect on detection results with this sensor.

Claims

1. A sensor for detecting or quantifying a target substance contained in a liquid sample, comprising:

a phenanthrenequinone and a phenanthrenequinone derivative as a mediator;

an enzyme dehydrogenating or oxidizing the target substance; and

at least a pair of electrodes;

wherein the amount of the phenanthrenequinone and the phenanthrenequinone derivative contained in the sensor is 0.05 to 2500 nmol per unit of the enzyme.

2. The sensor according to claim 1,

wherein the amount of the phenanthrenequinone and the phenanthrenequinone derivative contained in the sensor is 1 to 400 nmol per unit of the enzyme.