Method of measuring blood component, sensor used in the method, and measuring device

• US 9,213,012 B2
• Filed: 08/21/2013
• Issued: 12/15/2015
• Est. Priority Date: 12/04/2003
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: Fujiwara, Masaki, Shinno, Teppei, Ikeda, Shin
• Primary Examiner(s): Kaur, Gurpreet
• Application Number:
  US13/972,389
• Publication Number:
  US 20140158553A1
• Time in Patent Office: 846 Days
• Field of Search: 20440301-40315, 205/777.5, 205/778, 205/792, 600345-348, 436 62- 71, 436500-548
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/728 Bilirubin; including biliverdin

Abstract

The present invention provides a method of measuring a component in blood, by which an amount of the component can be corrected accurately by measuring a hematocrit (Hct) value of the blood with high accuracy and high reliability and also provides a sensor used in the method. The method of measuring a component in blood using a biosensor comprising a first electrode system including a first working electrode on which at least an oxidoreductase that acts upon the component and a mediator are provided and a first counter electrode and a second working electrode on which the mediator is not provided. The first working electrode and the first counter electrode are used for obtaining the amount of the component and the second working electrode and the first working electrode are used for obtaining the amount of the blood cells.

Claims

1. A method of measuring a component in blood using a biosensor comprising a first electrode system including a first working electrode on which at least an oxidoreductase that acts upon the component and a mediator are provided and a first counter electrode and a second working electrode on which the mediator is not provided, the method comprising:

a first step of obtaining a first current value by applying a first voltage to the first working electrode as a working electrode and the first counter electrode as a counter electrode; and

a second step of obtaining a second current value by applying a second voltage to the second working electrode as a working electrode and the first working electrode as a counter electrode,

wherein the first current value contains information on the amount of the component,

the second current value contains information related to an amount of blood cells in the blood,

the first voltage is applied for a time of 0.5 to 10 seconds and the second voltage is applied for a time of 0.001 to 10 seconds and

the first current value and the second current value are used to calculate a value of the amount of the component, corrected based on the amount of blood cells.

2. The method according to claim 1, wherein the first step is carried out prior to the second step.