Oscillating cell culture bioreactor
First Claim
1. A bioreactor comprisingone or more gas permeable, closed-loop chambers which can contain fluid and gases and a tissue engineering scaffold for cell or tissue culture;
- a tissue engineering scaffold or tissue engineering matrix secured directly to the wall of the closed-loop chamber or placed within a means for retaining the scaffold which is secured directly to the wall of closed-loop chamber so that fluid, cells and gases are forced through the entire thickness of the scaffold; and
an oscillator and controller for moving the gas permeable, closed-loop chamber bidirectionally along an axis horizontal to an axis normal to the closed-loop chamber to force cells and fluid within the gas permeable, closed-loop chamber by convective flow through the secured tissue engineering scaffold.
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Accused Products
Abstract
Methods and devices for cell or tissue culture are provided. One aspect provides a bioreactor having a gas permeable, closed-loop chamber for cell or tissue culture, and an oscillating means for moving the gas permeable, closed-loop chamber bidirectionally along an axis horizontal to an axis normal to the closed-loop chamber to force convection of cells and fluid in the gas permeable, closed-loop chamber. The bioreactor optionally includes a tissue engineering scaffold, an inlet means, an outlet means, and integrated sensors. Another aspect provides a bioreactor having a plurality of gas permeable, closed-loop chambers for cell or tissue culture. Methods of culturing cells and producing tissue constructs are also provided.
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Citations
19 Claims
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1. A bioreactor comprising
one or more gas permeable, closed-loop chambers which can contain fluid and gases and a tissue engineering scaffold for cell or tissue culture; -
a tissue engineering scaffold or tissue engineering matrix secured directly to the wall of the closed-loop chamber or placed within a means for retaining the scaffold which is secured directly to the wall of closed-loop chamber so that fluid, cells and gases are forced through the entire thickness of the scaffold; and an oscillator and controller for moving the gas permeable, closed-loop chamber bidirectionally along an axis horizontal to an axis normal to the closed-loop chamber to force cells and fluid within the gas permeable, closed-loop chamber by convective flow through the secured tissue engineering scaffold. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for culturing cells comprising
culturing the cells in a bioreactor comprising one or more gas permeable, closed-loop chambers which can contain fluid and gases and a tissue engineering scaffold for cell or tissue culture; -
a tissue engineering scaffold or tissue engineering matrix secured directly to the wall of the closed-loop chamber or placed within a means for retaining the scaffold which is secured directly to the wall of the closed-loop chamber so that fluid, cells and gases are forced through the entire thickness of the scaffold; and an oscillator and controller for moving the gas permeable, closed-loop chamber bidirectionally along an axis horizontal to an axis normal to the closed-loop chamber to force cells and fluid within the gas permeable, closed-loop chamber by convective flow through the secured tissue engineering scaffold. - View Dependent Claims (17, 18, 19)
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Specification