Methods of treating ischemic spasticity
First Claim
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1. A method of treating spinal cord injury in a subject in need thereof comprising:
- a) obtaining neural stem cells of human origin;
b) expanding in vitro the neural stem cells in a dispersed adherent culture to form an expanded population of neural stem cells, wherein expanding the neural stem cells comprises;
i.) providing at least one extracellular protein to a culture vessel;
ii.) culturing the dissociated neural stem cells in said culture vessel in the absence of serum;
iii.) adding to the culture vessel at least one growth factor; and
iv.) passaging the cultured cells prior to confluence;
c) concentrating the expanded population of neural stem cells to form concentrated neural stem cells; and
d) injecting a therapeutically effective amount of said concentrated neural stem cells into one or more areas of a spinal cord of the subject.
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Abstract
The invention relates generally to methods of treating spasticity, rigidity, or muscular hyperactivity conditions by introducing a portion of an expanded population of neural stem cells into an area of a recipient spinal cord.
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Citations
18 Claims
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1. A method of treating spinal cord injury in a subject in need thereof comprising:
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a) obtaining neural stem cells of human origin; b) expanding in vitro the neural stem cells in a dispersed adherent culture to form an expanded population of neural stem cells, wherein expanding the neural stem cells comprises; i.) providing at least one extracellular protein to a culture vessel; ii.) culturing the dissociated neural stem cells in said culture vessel in the absence of serum; iii.) adding to the culture vessel at least one growth factor; and iv.) passaging the cultured cells prior to confluence; c) concentrating the expanded population of neural stem cells to form concentrated neural stem cells; and d) injecting a therapeutically effective amount of said concentrated neural stem cells into one or more areas of a spinal cord of the subject. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification