Microfluidic element for analysis of a sample liquid

• US 9,221,051 B2
• Filed: 04/29/2013
• Issued: 12/29/2015
• Est. Priority Date: 10/29/2010
• Status: Active Grant

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Boehm, Christoph, Wuerl, Susanne
• Primary Examiner(s): Jarrett, Lore
• Application Number:
  US13/872,686
• Publication Number:
  US 20130243664A1
• Time in Patent Office: 974 Days
• Field of Search: 422/506
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: F16K 2099/0084 Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 99/0017 Capillary or surface tension valves, e.g. using electro-wetting or electro-capillarity effects
• CPC Class Codes: F16K 99/0063 using centrifugal forces

Abstract

A microfluidic element for analysis of a fluid sample having a substrate and a microfluidic transport system having a channel structure enclosed by the substrate and a covering layer. The channel structure comprises a channel with two side walls as well as a chamber that is in fluid communication with the channel. The chamber has a chamber wall with an inlet orifice. The channel comprises a channel section and a valve section adjoining the channel section, wherein the valve section is in fluid communication with the inlet orifice in the chamber wall in such a way that a fluid can flow from the channel through the valve section and into the chamber. The valve section has a fluid transport cross-section, which enlarges in flow direction. The fluid transport cross-section in the valve section is greater than the fluid transport cross-section in the preceding channel section.

Claims

1. A microfluidic element for analyzing a fluid sample, said microfluidic element comprising a substrate and a microfluidic transport system having a channel structure, the microfluidic transport system being enclosed by the substrate and a covering layer, wherein

the channel structure comprises a channel with two side walls, and a chamber which adjoins the channel,

the channel comprises a channel section and a valve section adjoining the channel section,

the two side walls run parallel to each other in the channel section,

the chamber has a chamber wall with an inlet orifice in which area of the chamber wall is greater than area of the inlet orifice in the same plane, and the chamber has a chamber side wall that adjoins the chamber wall,

the inlet orifice in the chamber wall adjoins the valve section, so that a fluid can flow out of the channel through the inlet orifice of the chamber wall and into the chamber,

the valve section is provided with a fluid transport cross-section that enlarges in width in a flow direction, wherein in width, the fluid transport cross-section of the valve section at the inlet orifice is larger than a fluid transport cross-section in the channel section at a transition from the channel section to the valve section, and wherein the fluid transport cross-section of the valve section enlarges continuously from the transition to the inlet orifice, and

an end of the valve section, which has dimensions which differ from the chamber, forms a capillary stop such that a fluid flowing through the channel is stopped at the end of the valve section at the inlet orifice of the chamber, and wherein the valve section has a length l, which is at least three times a distance b between side walls of the valve section at the transition from the channel section to the valve section.

2. The microfluidic element according to claim 1, wherein, perpendicular to the chamber wall and at a distance of 0.2 mm from the inlet orifice, the fluid transport cross-section of the chamber is at least 1.5 times greater than the fluid transport cross-section of the end of the valve section at the inlet orifice.

3. The microfluidic element according to claim 2, wherein, perpendicular to the chamber wall and at a distance of 0.2 mm from the inlet orifice, the fluid transport cross-section of the chamber is at least double the fluid transport cross-section of the end of the valve section at the inlet orifice.

4. The microfluidic element according to claim 2, wherein, perpendicular to the chamber wall and at a distance of 0.2 mm from the inlet orifice, the fluid transport cross-section of the chamber is at least four times greater than the fluid transport cross-section of the end of the valve section at the inlet orifice.

5. The microfluidic element according to claim 1, wherein a distance between side walls in the end of the valve section at the inlet orifice of the chamber is at least double the distance between the side walls in the channel section.

6. The microfluidic element according to claim 1, wherein a distance between side walls in the end of the valve section at the inlet orifice of the chamber is at least three times greater than the distance between the side walls in the channel section.

7. The microfluidic element according to claim 1, wherein a distance between side walls in the end of the valve section at the inlet orifice of the chamber is at least four times greater than the distance between the side walls in the channel section.

8. The microfluidic element according to claim 1, wherein the length l is at least five times the distance b.

9. The microfluidic element according to claim 1, wherein the length l is at least seven times the distance b.

10. The microfluidic element according to claim 1, wherein the length l corresponds to at most ten times the distance b.

11. The microfluidic element according to claim 1, wherein the valve section has a constant height h or a height that increases towards the inlet orifice of the chamber.

12. The microfluidic element according to claim 1, wherein the chamber comprises a ramp that extends away from the inlet orifice of the chamber wall in a manner such that its height reduces with increasing distance from the chamber wall.

13. The microfluidic element according to claim 12, wherein the width of the ramp is greater than the width of the inlet orifice.

14. The microfluidic element according to claim 12, wherein the width of the ramp is at least 10% greater than the width of the inlet orifice.

15. The microfluidic element according to claim 12, wherein the width of the ramp is at least double the width of the inlet orifice.

16. The microfluidic element according to claim 12, wherein the width of the ramp is at least three times greater than the width of the inlet orifice.

17. The microfluidic element according to claim 12, wherein the width of the ramp is at most five times greater than the width of the inlet orifice.

18. The microfluidic element according to claim 1, wherein the end of the valve section is positioned at the chamber wall such that a side wall of the valve section is positioned close to the chamber side wall such that the distance between the side wall of the valve section and the chamber side wall is at most equal to the width of the channel section.

19. The microfluidic element according to claim 18, wherein the distance between the side wall of the valve section positioned close to the chamber side wall and the chamber side wall is at most equal to half the width of the channel section.

20. The microfluidic element according to claim 18, wherein the distance between the side wall of the valve section positioned close to the chamber side wall and the chamber side wall is at most equal to one third of the width of the channel section.

21. The microfluidic element according to claim 1, wherein a rib is positioned in the valve section between two valve section side walls such that two adjacent valve section sub-channels are formed, which both end at the chamber wall of the chamber and in which one of the valve section sub-channels is narrower than the other, wherein the distance of the first valve section sub-channel from the second valve section sub-channel at the chamber wall is at least twice the width of the narrower valve section sub-channel.

22. The microfluidic element according to claim 21, wherein the distance of the first valve section sub-channel from the second valve section sub-channel at the chamber wall is at least four times larger than the width of the narrower valve section sub-channel.

23. The microfluidic element according to claim 21, wherein the distance of the first valve section sub-channel from the second valve section sub-channel at the chamber wall is at least six times larger than the width of the narrower valve section sub-channel.

24. The microfluidic element according to claim 21, wherein the two adjacent valve section sub-channels each form a capillary stop with the chamber configured as a geometric valve.

25. The microfluidic element according to claim 21, wherein the rib is positioned such that the two sub-channels form an angle of at least 40 degrees.

26. The microfluidic element according to claim 21, wherein the rib is positioned such that the two sub-channels form an angle of at least 50 degrees.

27. The microfluidic element according to claim 21, wherein the rib is positioned such that the two sub-channels form an angle of at least 60 degrees.

28. The microfluidic element according to claim 21, wherein the first sub-channel is a main sub-channel and the second sub-channel is a bypass sub-channel.

29. The microfluidic element according to claim 28, wherein the bypass sub-channel is wider than the main sub-channel.

30. The microfluidic element according to claim 28, wherein side walls of the main sub-channel run parallel to each other.

31. The microfluidic element according to claim 28, wherein the main sub-channel is aligned with the channel section.

32. The microfluidic element according to claim 28, wherein the chamber comprises a chamber side wall adjoining the chamber wall, and the bypass sub-channel of the valve section is positioned closer to the chamber side wall than the main sub-channel of the valve section.

33. The microfluidic element according to claim 28, wherein the height of the bypass sub-channel at a bypass inlet orifice of the chamber is greater than the height of the main sub-channel at the inlet orifice of the chamber.

34. The microfluidic element according to claim 33, wherein the heights of the bypass sub-channel and the main sub-channel at the transition to the channel section are equal.

35. The microfluidic element according to claim 33, wherein the channel structure of the microfluidic transport system is formed in the substrate.

36. A microfluidic element for analyzing a fluid sample, said microfluidic element comprising a substrate and a microfluidic transport system having a channel structure, the microfluidic transport system being enclosed by the substrate and a covering layer, wherein

the channel structure comprises a channel with two side walls, and a chamber which adjoins the channel,

the channel comprises a channel section and a valve section adjoining the channel section,

the two side walls run parallel to each other in the channel section,

the chamber has a chamber wall with an inlet orifice in which area of the chamber wall is greater than area of the inlet orifice in the same plane, and the chamber has a chamber side wall that adjoins the chamber wall,

the inlet orifice in the chamber wall adjoins the valve section, so that a fluid can flow out of the channel through the inlet orifice of the chamber wall and into the chamber,

the valve section is provided with a fluid transport cross-section that enlarges in width in a flow direction, wherein in width, the fluid transport cross-section of the valve section at the inlet orifice is larger than a fluid transport cross-section in the channel section at a transition from the channel section to the valve section, and wherein the fluid transport cross-section of the valve section enlarges continuously from the transition to the inlet orifice, and

an end of the valve section, which has dimensions which differ from the chamber, forms a capillary stop such that a fluid flowing through the channel is stopped at the end of the valve section at the inlet orifice of the chamber, and wherein

a rib is positioned in the valve section between two valve section side walls such that two adjacent valve section sub-channels are formed, which both end at the chamber wall of the chamber and in which one of the valve section sub-channels is narrower than the other, wherein the distance of the first valve section sub-channel from the second valve section sub-channel at the chamber wall is at least twice the width of the narrower valve section sub-channel.