Methods and compositions for modification of the HPRT locus
First Claim
1. A non-naturally occurring fusion protein comprising a zinc finger protein that binds to an endogenous hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene and a nuclease cleavage domain, wherein the fusion protein modifies the endogenous HPRT gene and further wherein the zinc finger protein comprises 5 or 6 zinc finger domains, each zinc finger domain comprising a recognition helix region, wherein the zinc finger protein comprises the recognition helix regions in the order shown in a single row of rows 3 to 5 or 10 to 23 of Table 1 and further wherein the zinc finger protein targets a sequence selected from the group consisting of SEQ ID NO:
- 41, 42 or 125-136.
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Accused Products
Abstract
Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals.
71 Citations
29 Claims
- 1. A non-naturally occurring fusion protein comprising a zinc finger protein that binds to an endogenous hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene and a nuclease cleavage domain, wherein the fusion protein modifies the endogenous HPRT gene and further wherein the zinc finger protein comprises 5 or 6 zinc finger domains, each zinc finger domain comprising a recognition helix region, wherein the zinc finger protein comprises the recognition helix regions in the order shown in a single row of rows 3 to 5 or 10 to 23 of Table 1 and further wherein the zinc finger protein targets a sequence selected from the group consisting of SEQ ID NO:
Specification