Devices and methods for manipulating deformable fluid vessels

• US 9,222,623 B2
• Filed: 03/12/2014
• Issued: 12/29/2015
• Est. Priority Date: 03/15/2013
• Status: Active Grant

• Current Assignee: Roche Molecular Systems Inc (Roche Holding AG)
• Original Assignee: Genmark Diagnostics Incorporated (Roche Holding AG)
• Inventors: Wright, David Walter, Aiello, Dominic
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): KRCHA, MATTHEW D
• Application Number:
  US14/206,867
• Publication Number:
  US 20140261708A1
• Time in Patent Office: 657 Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: B01L 3/523 with means for closing or opening
• CPC Class Codes: B65D 35/28 for expelling contents
• CPC Class Codes: B65D 35/30 Pistons
• CPC Class Codes: B65D 35/56 Holders for collapsible tubes B65D51/249 takes precedence
• CPC Class Codes: B65D 83/0055 Containers or packages provided with a flexible bag or a deformable membrane or diaphragm for expelling the contents
• CPC Class Codes: F17D 1/08 for liquids or viscous products water-main or service pipe systems E03B7/04; domestic hot-water supply systems F24D17/00
• CPC Class Codes: Y10T 137/0318 : Processes
• CPC Class Codes: Y10T 137/87917 : Flow path with serial valves and/or closures

Abstract

A fluid container comprises a first vessel, a second vessel connected or connectable to the first vessel, and a sealing partition preventing fluid flow from the second vessel. The container further includes a spherical opening element initially supported within the second vessel by the sealing partition and configured to be contacted with the sealing partition to open the sealing partition and permit fluid flow from the second vessel.

Claims

1. A fluid container comprising:

a first vessel;

a second vessel connected or connectable to the first vessel;

a sealing partition preventing fluid flow from the second vessel; and

a solid spherical opening element initially supported within the second vessel by the sealing partition and configured to be contacted with the sealing partition to open the sealing partition and permit fluid flow from the second vessel,

wherein the first vessel comprises a first collapsible blister supported on a planar substrate, and the second vessel comprises a second collapsible blister supported on the substrate.

2. The fluid container of claim 1, further comprising a fluid channel extending between the first and second vessels.

3. The fluid container of claim 2, further comprising a seal within the fluid channel, the seal being configured to be breakable upon application of sufficient force to the seal to thereby connect the first and second vessels via the fluid channel.

4. The fluid container of claim 1, further comprising a fluid channel formed in the substrate and extending between the first and second vessels.

5. The fluid container of claim 1, further comprising an opening formed in the substrate below the second vessel, wherein the sealing partition is disposed over the opening and the solid spherical opening element is disposed within the second vessel and supported above the opening on the sealing partition.

6. The fluid container of claim 5, wherein the solid spherical opening element is configured to be contacted with the sealing partition by collapsing the second vessel and pushing the solid spherical opening element through the sealing partition and into the opening.

7. The fluid container of claim 1, wherein the solid spherical opening element comprises a steel ball.

8. The fluid container of claim 1, wherein the sealing partition comprises a foil.

9. The fluid container of claim 6, further comprising a channel formed in the substrate and extending from the opening to permit fluid flowing from the second vessel and into the opening to flow through the channel.

10. A method for displacing fluid from a fluid container including a first vessel and a second vessel connected or connectable to the first vessel and including a sealing partition preventing fluid flow from the second vessel, wherein the fluid container further includes a solid spherical opening element disposed within the second vessel, said method comprising:

(a) applying a compressive force to the second vessel sufficient to collapse the second vessel and push the solid spherical opening element disposed within the second vessel into the sealing partition with sufficient force to rupture the sealing partition to thereby permit fluid flow form the second vessel; and

(b) applying a compressive force to the first vessel sufficient to collapse the first vessel and force fluid from the first vessel to the second vessel, whereby fluid forced into the second vessel flows out of the second vessel through the ruptured sealing partition.

11. The method of claim 10, wherein the second vessel is supported on a substrate which comprises an opening formed in the substrate below the second vessel and wherein the sealing partition is disposed over the opening and the solid spherical opening element is supported above the opening on the sealing partition, and step (a) comprises pushing the solid spherical opening element through the sealing partition and into the opening formed in the substrate below the second vessel.

12. The method of claim 10, wherein step (a) is performed with a first external actuator configured to apply a compressive force to the second vessel, and step (b) is performed with a second external actuator configured to apply a compressive force to the first vessel.

13. The method of claim 10, wherein step (b) is performed after step (a) is performed.

14. The method of claim 10, wherein the fluid container includes a fluid channel extending between the first vessel and the second vessel with a fluid-blocking seal within the fluid channel, and wherein step (b) comprises applying sufficient force to alter the seal and thereby connect the first and second vessels via the fluid channel.