Nano-porous membrane based sensors

• US 9,222,907 B2
• Filed: 04/02/2013
• Issued: 12/29/2015
• Est. Priority Date: 12/02/2005
• Status: Expired due to Fees

• Current Assignee: State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Portland State University
• Original Assignee: State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Portland State University
• Inventors: Prasad, Shalini, Kondama Reddy, Ravi Kiran
• Primary Examiner(s): LEONG, SUSAN DANG
• Application Number:
  US13/855,146
• Publication Number:
  US 20130224765A1
• Time in Patent Office: 1,001 Days
• Field of Search: 20440301-40315, 205/81, 205/792, 205/777.5, 205/778, 436 62- 71, 436500-548
• US Class Current: 1/1
• CPC Class Codes: G01N 27/26 by investigating electrochemical variables; by using electrolysis or electrophoresis
• CPC Class Codes: G01N 27/3278 involving nanosized elements, e.g. nanogaps or nanoparticles nanopores G01N33/48721; magnetic beads G01N27/745

Abstract

Sensors include nano-porous alumina membranes that are sensitized by immobilization of antibodies in the nano-pores. The nano-membranes can be sensitized to respond to a single target compound, or different portions of the nano-membrane can be differently sensitized. Capture of the target compound can be detected based on a spectral signature associated with electrical conductance in the nano-pores.

Claims

1. A sensor, comprising:

a solid substrate having a top surface;

a nano-porous membrane situated at the top surface of the solid substrate, the membrane including a plurality of nano-pores that do not extend through the nano-porous membrane, the membrane including a first sensitizing agent for detection of a target compound, the first sensitizing agent being immobilized within a first set of nano-pores, thereby defining a sensitized portion of the membrane;

a first conductor electrically coupled to the first sensitizing agent immobilized in the first set of nano-pores defined in the membrane, the first conductor situated on the top surface of the solid substrate and in contact with the sensitized portion of the membrane, the first conductor outputting an electrical signal associated with detection of the target compound; and

an electrical signal detector for receiving the electrical signal to allow evaluation of the electrical signal following administration of a test specimen to the sensor.

2. The sensor of claim 1, further comprising:

a second conductor electrically coupled to a second set of nano-pores; and

a second sensitizing agent immobilized within the second set of nano-pores.

3. The sensor of claim 2, wherein the first sensitizing agent and the second sensitizing agent are the same.

4. The sensor of claim 2, wherein the first sensitizing agent and the second sensitizing agent are different.

5. The sensor of claim 1, wherein the detector is a spectrum analyzer that is in communication with the first conductor, the spectrum analyzer being configured to produce an estimate of a received signal portion based on a signature frequency or frequencies associated with specific binding of the target compound to the first sensitizing agent.

6. The sensor of claim 5, wherein the spectrum analyzer is configured to produce an estimate of a received signal portion associated with at least two frequencies associated with a detection signature.

7. The sensor of claim 2, wherein the detector is a spectrum analyzer that is selectively coupled to the first conductor and the second conductor, wherein the spectrum analyzer is configured to produce an estimate of a received signal portion associated with a frequency or frequencies associated with a first signature and a frequency or frequencies associated with a second signature.

8. The sensor of claim 7, wherein the spectrum analyzer is configured to produce an estimate of a received signal portion associated with at least two frequencies associated with the first signature.

9. The sensor according to claim 1 wherein the detector is a signature analyzer electrically coupled to the first conductor and configured to estimate a target compound concentration based on at least one signature portion associated with specific binding of the target compound to the sensitizing agent within the first set of nano-pores.

10. The sensor of claim 9, wherein the signature portion is a signal magnitude in a selected frequency range.

11. The sensor of claim 9, wherein the sensor is in communication with a signature database, and the signature analyzer is configured to produce an indication of a presence of at least one target compound based on a comparison of the at least one signature portion associated with specific binding of the target compound by the sensitizing agent with at least one signature stored in the signature database associated with specific binding.

21. The sensor of claim 7, wherein the spectrum analyzer is configured to produce an estimate of a received signal portion associated with at least two frequencies associated with the first signature and at least two frequencies associated with the second signature.

12. An apparatus, comprising:

a base substrate having a plurality of conductor segments defined thereon;

a nano-porous membrane secured to the base substrate, the nano-porous membrane including a plurality of sensitized nano-pores that do not extend through the nano-porous membrane, each nano-pore having a sensitizer for detection of a target compound, the sensitizer being immobilized within the nano-pore, and the sensitizer electrically coupled to a corresponding conductor segment which outputs an electrical signal associated with detection of the target compound;

a fluid chamber for receiving a test fluid and communicating the test fluid to the sensitized nano-pores; and

an electrical signal detector for receiving the electrical signal, thereby allowing evaluation of the electrical signal associated with administration of a test specimen to the sensor.

13. The apparatus of claim 12, wherein the nano-porous membrane is an alumina membrane having a plurality of nano-pores with effective diameters in a range of 5 nm to 500 nm.

14. The apparatus of claim 13, wherein the plurality of nano-pores comprises a first nano-pore having a first effective diameter, and a second nano-pore having a second effective diameter, and wherein the first effective diameter is larger than the second effective diameter.

15. The apparatus of claim 12, wherein the detector is a spectrum analyzer configured to receive electrical signals from the conductor segments and produce spectra of the electrical signals.

22. The apparatus of claim 12, further comprising a first set of sensitized nano-pores, the first set of nano-pores having a first sensitizer for detection of a first target compound.

23. The apparatus of claim 22, further comprising a second set of sensitized nano-pores, the second set of nano-pores having a second sensitizer for detection of a second target compound.

16. A method, comprising:

administering a test specimen to a sensor comprising a solid substrate having a top surface, a nano-porous membrane situated at the top surface of the solid substrate, the membrane including a plurality of nano-pores that do not extend through the nano-porous membrane, the membrane including a first sensitizing agent for detection of a target compound, the first sensitizing agent being immobilized within a first nano-pore so as to define a sensitized portion of the membrane, and a first conductor electrically coupled to the first sensitizing agent immobilized in the first nano-pore defined in the membrane, the first conductor situated on the top surface of the solid substrate and in contact with the sensitized portion of the membrane; and

evaluating an electrical signal detector for a detected electrical signal to determine presence or absence of the target compound in the test specimen.

17. The method of claim 16, further comprising:

evaluating a detected electrical signal to identify a magnitude of at least one electrical spectral peak associated with exposure of the sensor to the target compound; and

assessing the test specimen based on the magnitude.

18. The method of claim 16, wherein the test specimen is administered to a sensor sensitized to at least a first target compound and a second target compound.

19. The method of claim 16, wherein the target compound is prostate specific antigen.

24. The method of claim 16, wherein the first sensitizing agent is immobilized within a first set of nano-pores, and the first conductor is electrically coupled to the first sensitizing agent immobilized in the first set of nano-pores.

20. A method, comprising:

administering a test specimen to a sensor comprising a solid substrate having a top surface, a nano-porous alumina membrane situated at the top surface of the solid substrate, the membrane including a plurality of nano-pores that do not extend through the nano-porous membrane, the membrane including a first sensitizing agent for detection of a target compound, the first sensitizing agent being immobilized within a first set of nano-pores so as to define a sensitized portion of the membrane, and a first conductor electrically coupled to the first sensitizing agent immobilized in the first set of nano-pores defined in the membrane, the first conductor situated on the top surface of the solid substrate and in contact with the sensitized portion of the membrane;

evaluating an electrical signal associated with administration of the test specimen to the sensor; and

assessing the test specimen based on the evaluation.