Unitized reagent strip

• US 9,222,954 B2
• Filed: 03/27/2014
• Issued: 12/29/2015
• Est. Priority Date: 09/30/2011
• Status: Active Grant

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Lentz, Ammon David, St. Pierre, Richard, Livingston, Dwight, Steel, Adam Bruce
• Primary Examiner(s): Mui, Christine T
• Application Number:
  US14/227,883
• Publication Number:
  US 20140206088A1
• Time in Patent Office: 642 Days
• Field of Search: 436/54, 436/43, 422/549, 422/550, 422/554, 422/500, 422/50, 435/304.1, 435/289.1, 435/283.1
• US Class Current: 1/1
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B01L 3/527 for a plurality of reagents
• CPC Class Codes: B01L 9/543 for disposable pipette tips, e.g. racks or cassettes
• CPC Class Codes: G01N 2035/103 using disposable tips
• CPC Class Codes: G01N 35/10 Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence
• CPC Class Codes: G01N 35/1011 Control of the position or alignment of the transfer device
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure

Abstract

The embodiments disclosed herein relate to unitized reagent strips for holding and transporting reagents and materials used in automated sample preparation and/or processing for biological and or chemical assays.

Claims

1. A method of detecting the presence or absence of a pipette tip within a pipette sheath of a unitized reagent strip, comprising:

providing the unitized reagent strip, the unitized reagent strip comprising;

a strip with a top side and a bottom side, comprising

a first and a second pipette sheath, the first pipette sheath comprising opposing sides and a first longitudinal axis, said first and second pipette sheaths comprising a first and second pipette tip aperture, respectively, each of which comprises a separate opening on the top side of the strip, and wherein said first and second pipette tip apertures are configured for insertion of a first and second pipette tip into said first and second pipette sheaths, respectively, and wherein each of said first and second pipette sheaths is configured to substantially surround the length of the first and second pipette tip, respectively;

a process tube; and

a receptacle, comprising an opening through the reagent strip, wherein said receptacle is configured to receive a reagent tube,

wherein said first pipette sheath comprises an aperture pair, said aperture pair comprising a first cored hole and a second cored hole, wherein the first and second cored holes are located on the opposing sides, and are positioned along the th of the first pipette sheath at the same distance from the first pipette tip aperture, the first and second cored holes having an axis transverse to the first longitudinal axis,

providing an optical beam through the first cored hole of said aperture pair; and

detecting whether said optical beam exits unobstructed through said second cored hole of said aperture pair,

determining whether the first pipette tip is present within the first pipette sheath, wherein the unobstructed exit of said optical beam through said second cored hole of said first pipette sheath is indicative of the absence of the first pipette tip within the first pipette sheath, and wherein obstructed exit of said optical beam though said second cored hole indicates the presence of the first pipette tip within said first pipette sheath.

2. The method of claim 1, wherein the first pipette sheath comprises a second aperture pair, wherein the second aperture pair is a different distance along the length of the first pipette sheath, the method further comprising determining the length of the first pipette tip inserted within the first pipette sheath.

3. The method of claim 1, wherein the first pipette sheath comprises multiple cored holes, the method further comprising determining the length of the first pipette tip inserted within the first pipette sheath.

4. The method of claim 1, wherein the first pipette tip alters the reflection or the obstruction of light directed through the first cored hole but does not alter the reflection or the obstruction of light directed through another cored hole.

5. The method of claim 1, wherein determining whether the first pipette tip is present within the first pipette sheath further comprises using optical sensors.

6. A method of determining the length of a pipette tip within a pipette sheath of a unitized reagent strip, comprising:

providing a unitized reagent strip comprising;

a strip with a top side and a bottom side, comprising;

a process tube;

a receptacle, comprising an opening through the reagent strip, wherein said receptacle is configured to receive a reagent tube; and

a first and a second pipette sheath, comprising;

a first and second pipette tip aperture, respectively, each of which comprises a separate opening on the top side of the strip, and wherein said first and second pipette tip apertures are configured for insertion of a first and second pipette tip into said first and second pipette sheaths, respectively, and wherein each of said first and second pipette sheaths is configured to substantially surround the length of the first and second pipette tip, respectively; and

a top pipette sheath aperture pair and a bottom pipette sheath aperture pair within said first pipette sheath, said top and bottom pipette sheath aperture pairs each comprising a first and a second cored hole extending through a sidewall of the first pipette sheath, wherein the first and second cored holes of said top and bottom pipette sheath aperture pairs are located on opposite sides of the first pipette sheath, and positioned along the length of the first pipette sheath at the same distance from the first pipette tip aperture, and wherein said top pipette sheath aperture pair is located more proximal to the first pipette tip aperture than said bottom pipette sheath aperture pair;

providing an optical beam through said first cored hole of said top pipette sheath aperture pair;

providing an optical beam through said first cored hole of said bottom pipette sheath aperture pair;

detecting whether said optical beam is obstructed from passing through said second cored hole of said top pipette sheath aperture pair; and

detecting whether said optical beam is obstructed from passing through said second cored hole of said bottom pipette sheath aperture pair, wherein obstruction of said optical beam through the second cored hole of the top pipette sheath aperture pair and passage of said optical beam through said second cored hole of said bottom pipette sheath aperture pair indicates that the first pipette tip within said first pipette sheath has a length that does not extend down to the bottom pipette sheath aperture pair when inserted into the first pipette sheath.

7. The method of claim 6, wherein obstruction of said optical beam through the second cored hole of the top pipette sheath aperture pair and obstruction of said optical beam through said second cored hole of said bottom pipette sheath aperture pair indicates that the first pipette tip within said first pipette sheath has a length that extends down to the bottom pipette sheath aperture pair when inserted into the first pipette sheath.

8. The method of claim 6, wherein obstruction of said optical beam through the second cored hole of the top pipette sheath aperture pair indicates that the first pipette tip is present within said first pipette sheath.

9. A method of detecting the presence or absence of a pipette tip within a pipette sheath of a unitized reagent strip, comprising:

providing the unitized reagent strip, the unitized reagent strip comprising;

a strip with a top side and a bottom side, comprising;

a first and a second pipette sheath comprising opposing sides, said first and second pipette sheaths comprising a first and second pipette tip aperture, respectively, each of which comprises a separate opening on the top side of the strip, and wherein said first and second pipette tip apertures are configured for insertion of a first and second pipette tip into said first and second pipette sheaths, respectively, and wherein each of said first and second pipette sheaths is configured to substantially surround the length of the first and second pipette tip, respectively,

a process tube; and

a receptacle, comprising an opening through the reagent strip, wherein said receptacle is configured to receive a reagent tube,

wherein the first pipette sheath comprises a first cored hole, said first cored hole extending through a sidewall of the first pipette sheath,

determining whether the first pipette tip extends within said first pipette sheath from the first pipette tip aperture at least to the distance of the first cored hole,

wherein said determining step comprises;

providing an optical beam that enters the first pipette sheath through the first cored hole, and

determining the reflection or obstruction of the optical beam as indicative of the presence or absence of the first pipette tip that extends within said first pipette sheath to the distance from the first pipette tip aperture to the first cored hole.

10. The method of claim 9, wherein said determining step comprises visual inspection of the interior of the first pipette sheath through the first cored hole.

11. The method of claim 9, wherein the first pipette sheath comprises an alternative cored hole, wherein the alternative cored hole is a different distance along the length of the first pipette sheath than the first cored hole, the method further comprising determining the length of the first pipette tip inserted within the first pipette sheath.

12. The method of claim 9, wherein the first pipette sheath comprises multiple cored holes, the method further comprising determining the length of the first pipette tip inserted within the first pipette sheath.

13. The method of claim 9, wherein the first pipette tip alters the reflection or the obstruction of light directed through the first cored hole but does not alter the reflection or the obstruction of light directed through another cored hole.

14. The method of claim 9, wherein the first pipette sheath comprises at least two cored holes, the method further comprising determining the reflection or obstruction of the optical beam as indicative of the presence or absence of the first pipette tip that extends within said first pipette sheath to the distance from the first pipette tip aperture to the at least two cored holes.

15. A method of determining the length of a pipette tip within a pipette sheath of a unitized reagent strip, comprising:

providing a unitized reagent strip comprising;

a strip with a top side and a bottom side, comprising;

a process tube; and

a receptacle, comprising an opening through the reagent strip, wherein said receptacle is configured to receive a reagent tube; and

a first and a second pipette sheath, comprising;

a first and second pipette tip aperture, respectively, each of which comprises a separate opening on the top side of the strip, and wherein said first and second pipette tip apertures are configured for insertion of a first and second pipette tip into said first and second pipette sheaths, respectively, and wherein each of said first and second pipette sheaths is configured to substantially surround the length of the first and second pipette tip, respectively; and

a top cored hole and a bottom cored hole within said first pipette sheath, said top and bottom cored holes each extending through a sidewall of the first pipette sheath, wherein said top cored hole is located more proximal to the first pipette tip aperture than said bottom cored hole;

determining whether the first pipette tip extends within said first pipette sheath from the first pipette tip aperture to the distance of the top cored hole; and

determining whether the first pipette tip extends within said first pipette sheath from the first pipette tip aperture to the distance of the bottom cored hole.

16. The method of claim 15, wherein said determining comprises visual inspection of the interior of the first pipette sheath through said top cored hole and said bottom cored hole.

17. The method of claim 15, wherein said determining comprises:

providing an optical beam through said top cored hole;

providing an optical beam through said bottom cored hole; and

determining the reflection or obstruction of the first and second optical beam as indicative of the presence or absence of the first pipette tip that extends within said first pipette sheath to the distance from the first pipette tip aperture to the top cored hole and the bottom cored hole, respectively.

18. The method of claim 15, further comprising determining whether the first pipette tip is present within the first pipette sheath.

19. The method of claim 15, wherein determining whether the first pipette tip extends within said first pipette sheath from the first pipette tip aperture to the distance of the top cored hole comprises passing a beam of light through the top cored hole.

20. The method of claim 15, wherein determining whether the first pipette tip extends within said first pipette sheath from the first pipette tip aperture to the distance of the bottom cored hole comprises passing a beam of light through the bottom cored hole.