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Stabilized fibronectin domain compositions, methods and uses

  • US 9,234,029 B2
  • Filed: 09/23/2013
  • Issued: 01/12/2016
  • Est. Priority Date: 04/30/2010
  • Status: Active Grant
First Claim
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1. A protein scaffold comprising loop domains topologically similar to loop domains of a fibronectin type III (FN3) domain, the protein scaffold having an amino acid sequence based on the consensus amino acid sequence of SEQ ID NO:

  • 16, wherein one or more specific residues of SEQ ID NO;

    16 are replaced to enhance melting temperature (Tm) and chemical stability of the protein scaffold as compared to the Tm and chemical stability of a protein scaffold having the amino acid sequence of SEQ ID NO;

    16, and the replacements of SEQ ID NO;

    16 are selected from the group consisting of N46V, E14P, L17A, E86I, a combination of N46V and E86I, a combination of N46V and E86I, and a combination of L17A, N46V and E86I.

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