System for management of yeast to facilitate the production of ethanol
First Claim
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1. A method of propagating ethanologen for use in the production of a fermentation product from biomass comprising the steps of:
- providing a medium for propagation of ethanologen;
supplying a first cell mass of ethanologen to the medium;
supplying xylose to the medium as a carbon source for cell mass growth of the ethanologen;
maintaining the medium comprising the first cell mass of ethanologen at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen;
aerating the medium so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen;
and wherein, the second cell mass of ethanologen is at least 200 times larger than the first cell mass of ethanologen.
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Abstract
A system and method for managing an ethanologen for use in biorefinery is disclosed. The method for propagating ethanologen for use in the production of a fermentation product from biomass comprises the steps of providing a medium for propagation of ethanologen and supplying a first cell mass of ethanologen to the medium. A first cell mass of ethanologen is propagated into a larger second cell mass of ethanologen.
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Citations
21 Claims
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1. A method of propagating ethanologen for use in the production of a fermentation product from biomass comprising the steps of:
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providing a medium for propagation of ethanologen; supplying a first cell mass of ethanologen to the medium; supplying xylose to the medium as a carbon source for cell mass growth of the ethanologen; maintaining the medium comprising the first cell mass of ethanologen at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen; aerating the medium so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen; and wherein, the second cell mass of ethanologen is at least 200 times larger than the first cell mass of ethanologen. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method of propagating ethanologen for use in the production of a fermentation product from biomass comprising the steps of:
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providing a medium for propagation of ethanologen; supplying a first cell mass of ethanologen to the medium; providing an agent to the medium; providing a component obtained from the biomass to the medium, wherein the component comprises xylose as a carbon source for the ethanologen; maintaining the medium comprising the first cell mass of ethanologen at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen; aerating the medium so that the first cell mass of ethanologen is propagated into a second cell mass of ethanologen; wherein the second cell mass of ethanologen is at least 200 times larger than the first cell mass of ethanologen; wherein the biomass comprises lignocellulosic material; wherein the lignocellulosic material comprises at least one of corn cobs, corn plant husks, corn plant leaves and corn plant stalks; wherein the component is obtained from the lignocellulosic material; and wherein the ethanologen comprises yeast cells capable of fermenting xylose into ethanol. - View Dependent Claims (18, 19)
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20. A system for propagating ethanologen for use in the production of a fermentation product from biomass in a fermentation system comprising:
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a first stage comprising a first vessel configured to contain a medium comprising ethanologen; a second stage comprising a second vessel configured to contain a medium supplied from the first stage; a source of xylose to be provided to the medium as a carbon source for ethanologen in the first stage; a source of xylose to be provided to the medium as a carbon source for the ethanologen in the second stage; wherein the ethanologen has a first cell mass when supplied to the first stage and the ethanologen has a second cell mass at least 200 times larger than the first cell mass when supplied from the first stage to the second stage and the ethanologen has a third cell mass at least 20 times larger than the second cell mass when supplied from the second stage; wherein the first vessel is configured to aerate the medium and maintain the medium at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius so that ethanologen can be propagated into the second cell mass; and wherein the second vessel is configured to aerate the medium and maintain the medium at a pH of between about 5.0 and 6.0 and at a temperature of between about 26 and about 37 degrees Celsius so that ethanologen can be propagated into the third cell mass. - View Dependent Claims (21)
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Specification