Regulating stem cells
First Claim
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1. A method of making a composition comprising:
- obtaining an initiating cell population (ICP) of at least ten thousand cells that have a density of less than 1.072 g/ml and at least 25% of which are CD31Bright; and
in vitro stimulating the ICP by culturing the ICP in the presence of one or more factors selected from the group consisting of;
autologous serum, vascular endothelial growth factor (VEGF), heparin, insulin-like growth factor (IGF), basic fibroblast growth factor (b-FGF), epidermal growth factor (EGF), and ascorbic acid, to differentiate into a progenitor/precursor cell population (PCP) comprising a subpopulation of cells that stain as CD31Bright, demonstrate uptake of Ac-LDL+ and secrete interleukin-8 (IL-8) and angiogenin, wherein the sub-population of cells comprises at least 10% of cells in the PCP, to provide for said composition.
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Abstract
Provided are methods for producing progenitor/precursor cells from a population of initiating cells (ICP) that have a density of less than 1.072 g/ml and at least 25% of which are CD31Bright by in vitro stimulating the ICP with different factors.
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Citations
18 Claims
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1. A method of making a composition comprising:
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obtaining an initiating cell population (ICP) of at least ten thousand cells that have a density of less than 1.072 g/ml and at least 25% of which are CD31Bright; and in vitro stimulating the ICP by culturing the ICP in the presence of one or more factors selected from the group consisting of;
autologous serum, vascular endothelial growth factor (VEGF), heparin, insulin-like growth factor (IGF), basic fibroblast growth factor (b-FGF), epidermal growth factor (EGF), and ascorbic acid, to differentiate into a progenitor/precursor cell population (PCP) comprising a subpopulation of cells that stain as CD31Bright, demonstrate uptake of Ac-LDL+ and secrete interleukin-8 (IL-8) and angiogenin, wherein the sub-population of cells comprises at least 10% of cells in the PCP, to provide for said composition. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification