Human multipotent embryonic stem cell-like progenitor cells

• US 9,234,177 B2
• Filed: 10/18/2011
• Issued: 01/12/2016
• Est. Priority Date: 10/18/2010
• Status: Expired due to Fees

• Current Assignee: Sunshine Life Science & Technology Corp.
• Original Assignee: Sunshine Life Science & Technology Corp.
• Inventors: Tsai, Ming-Song, Tzu-Bi Shih, Daniel
• Primary Examiner(s): Gitomer, Ralph
• Assistant Examiner(s): CLARKE, TRENT R
• Application Number:
  US13/276,106
• Publication Number:
  US 20120094380A1
• Time in Patent Office: 1,547 Days
• US Class Current: 1/1
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/999 Small molecules not provided for elsewhere
• CPC Class Codes: C12N 5/0605 Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
• CPC Class Codes: C12N 5/0668 Mesenchymal stem cells from other natural sources
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties

Abstract

The invention provides a plurality of embryonic stem cell-like progenitor cells, which are isolated from a human tissue by a systemic screening of human mesenchymal stromal stem/progenitor cells and a cell sorting by a cell antigen selected from the group consisting of CD34, CD117, CD133, CD201, GloboH and combination thereof, and cultured in a medium supplemented with at least one or more steroids and one or more growth factors. The cells of the invention express CD34 and exhibit sphere-like clonogenicity in early passages and express multipotent embryonic stem cells (ESCs) like characteristics.

Claims

1. A method for producing an enriched population of multipotent human CD34⁺ mesenchymal common progenitor cells (CD34⁺ MCPCs), further having at least the following characteristics:

CD14⁺, CD117⁺, CD133⁺ (AC133⁺), CD201⁺, Nestin⁺, SSEA3⁺, SSEA4⁺ and GloboH⁺, the method comprising;

isolating from a human somatic tissue by a systemic screening of human mesenchymal stromal stem/progenitor cells followed by a cell sorting by a cell antigen of CD34, wherein the human somatic tissue is selected from the group consisting of amnion, endometrium and gingival; and

culturing in a medium comprising EGF and hydrocortisone or a medium comprising bFGF and EGF.

2. The method according to claim 1, wherein the cell sorting is a fluorescence-activated cell sorting (FACS) flow cytometry.