Materials and methods for generating pluripotent stem cells
First Claim
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1. A method for obtaining human induced pluripotent stem cells comprising culturing human adult skin fibroblast cells on a feeder layer of human amniotic epithelial cells for a period of time sufficient to reprogram the adult skin fibroblast cells into human induced pluripotent stem (iPS) cells.
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Abstract
The present invention relates to a method of reprogramming a somatic cell to produce an induced pluripotent stem (iPS) cell which is capable of differentiating into somatic cells derived from ectoderm, mesoderm or endoderm. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells.
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7 Claims
- 1. A method for obtaining human induced pluripotent stem cells comprising culturing human adult skin fibroblast cells on a feeder layer of human amniotic epithelial cells for a period of time sufficient to reprogram the adult skin fibroblast cells into human induced pluripotent stem (iPS) cells.
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