Topical composition comprising transformed bacteria expressing a compound of interest

• US 9,234,204 B2
• Filed: 08/07/2013
• Issued: 01/12/2016
• Est. Priority Date: 08/07/2012
• Status: Expired due to Fees

• Current Assignee: TopGeniX Inc.
• Original Assignee: TopGeniX Inc.
• Inventors: Qvit-Raz, Noga, Altman, Tahel
• Primary Examiner(s): Leith, Nancy J
• Application Number:
  US13/961,845
• Publication Number:
  US 20140044653A1
• Time in Patent Office: 888 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A61K 2800/592 Mixtures of compounds complementing their respective functions
• CPC Class Codes: A61K 35/744 Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
• CPC Class Codes: A61K 8/27 Zinc; Compounds thereof
• CPC Class Codes: A61K 8/40 containing nitrogen quinones containing nitrogen A61K8/355
• CPC Class Codes: A61K 8/99 from microorganisms other than algae or fungi, e.g. protozoa or bacteria
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/06 Antipsoriatics
• CPC Class Codes: A61P 17/16 Emollients or protectives, e.g. against radiation
• CPC Class Codes: A61P 17/18 Antioxidants, e.g. antiradicals preparations for protection against sunlight A61Q17/00
• CPC Class Codes: A61P 39/06 Free radical scavengers or antioxidants
• CPC Class Codes: A61Q 17/00 Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
• CPC Class Codes: A61Q 17/04 Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
• CPC Class Codes: A61Q 19/007 Preparations for dry skin
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations
• CPC Class Codes: C12N 15/746 for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)

Abstract

Compositions comprised of a population of transformed bacteria formulated for topical application to a subject are described. The population of transformed bacteria are created from a non-pathogenic bacteria and transformed to express a compound of interest for a therapeutic or a cosmetic purpose. In one embodiment, the composition is for protection of the skin from ultraviolet rays.

Claims

1. A method of providing protection to human skin from UVA and UVB radiation, from 280-400 nm, by topically applying to the skin non-pathogenic, Gram-positive bacteria that has been genetically modified to express a mycosporine-like amino acid (MAA), wherein the method comprises the steps of

(a) transforming non-pathogenic, Gram-positive bacteria selected from the group consisting of Lactobacillus lactis and Lactococcus lactis with a vector comprising a sequence encoding shinorine;

(b) expressing shinorine at a level of about 0.1 mM to about 100 mM for 10²-10²⁰ cfu of the transformed bacteria, and

(c) topically applying to the skin of a human in need of protection from UVA and UVB radiation at least 10² cfu/cm² of the transformed bacteria from step (a).

2. The method of claim 1, wherein prior to step (c) the transformed bacteria are attenuated or killed.

3. The method of claim 1 wherein the transformed bacteria is present in a topical composition at a concentration of at least 0.1% by weight of the total composition.

4. The method of claim 1 comprising the further step of applying to the skin avobenzone.

5. The method of claim 4 comprising the further step of applying to the skin a sunscreen active ingredient that absorbs UVB radiation.

6. The method of claim 5 wherein the sunscreen active ingredient that absorbs UVB radiation is octocrylene.

7. The method of claim 3 wherein the topical composition further comprises avobenzone.

8. The method of claim 7 wherein the topical composition further comprises a sunscreen active ingredient that absorbs UVB radiation.

9. The method of claim 8 wherein the sunscreen active ingredient that absorbs UVB radiation is octocrylene.

10. The method of claim 6 wherein the topical composition is an oil-in-water or water-in-oil emulsion.

11. The method of claim 1 comprising the further step of applying to the skin a photoprotective amount of zinc oxide.