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Detection and quantification process for more than one nucleic acid in library

  • US 9,234,234 B2
  • Filed: 07/27/2004
  • Issued: 01/12/2016
  • Est. Priority Date: 06/30/2001
  • Status: Expired due to Term
First Claim
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1. A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of:

  • a) providing;

    (i) an array of fixed or immobilized nucleic acids complementary to said nucleic acids of interest;

    (ii) a library of nucleic acid analytes which may contain the nucleic acids of interest sought to be detected or quantified;

    (iii) means for attaching one or more non-inherent heteropolymeric universal detection targets (UDT) to a nucleic acid, wherein said attaching means is selected from blunt-end ligation with DNA ligase, ligation of single-stranded ends with RNA ligase or a combination thereof;

    (iv) a universal detection element (UDE) which generates a signal directly or indirectly;

    b) attaching said non-inherent UDTs to said library of nucleic acid analytes;

    c) hybridizing said library of nucleic acid analytes from step b) with said array of fixed or immobilized nucleic acids to form hybrids if said nucleic acids of interest are present;

    d) contacting said UDE with said non-inherent UDTs to form a complex bound to said array; and

    e) detecting or quantifying said more than one nucleic acid of interest by detecting or measuring the amount of signal generated from any UDE bound to said array.

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