Techniques and droplet actuator designs for reducing bubble formation

• US 9,238,222 B2
• Filed: 11/20/2014
• Issued: 01/19/2016
• Est. Priority Date: 06/27/2012
• Status: Expired due to Fees

• Current Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated), Illumina France Sarl (Illumina Incorporated)
• Original Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated), Illumina France Sarl (Illumina Incorporated)
• Inventors: Delattre, Cyril, Srinivasan, Vijay, Rival, Arnaud
• Primary Examiner(s): Ball, J. Christopher
• Application Number:
  US14/549,123
• Publication Number:
  US 20150075991A1
• Time in Patent Office: 425 Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/06 Fluid handling related problems
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/088 Channel loops
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0442 thermal energy, e.g. vaporisation, bubble jet
• CPC Class Codes: B01L 2400/0448 Marangoni flow; Thermocapillary effect
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B01L 3/5029 using swabs
• CPC Class Codes: B01L 3/52 Containers specially adapted for storing or dispensing a reagent B01L3/02 takes precedence; containers for medical or pharmaceutical purposes A61J1/00; containers in general B65D; storing or dispensing test elements G01N33/4875; automated reagent dispensing G01N35/1002

Abstract

During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

Claims

1. A droplet actuator comprising:

a. a top substrate and a bottom substrate separated to form a droplet operations gap, wherein the droplet operations gap is filled with a filler fluid;

b. a sidewall and an opposite sidewall bounding the droplet operations gap, thereby creating a droplet operations channel;

c. an arrangement of droplet operations electrodes on the sidewall; and

d. an arrangement of one or more ground electrodes along the opposite sidewall, wherein the one or more ground electrodes are connected to an electrical ground;

wherein multiple droplet operations may be conducted on one or more droplets in the droplet operations gap while maintaining substantially consistent contact between the one or more droplets and the one or more ground electrodes, thereby permitting completion of the multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

2. The droplet actuator according to claim 1, wherein the sidewall comprises a first rail and the opposite sidewall comprises a second rail, wherein the first rail and second rail are elongated three-dimensional (3D) structures that are arranged in parallel with each other.

3. The droplet actuator according to claim 1, further comprising offsetting positions of the droplet operations electrodes and the position of the one or more ground electrodes.

4. The droplet actuator according to claim 1, wherein the one or more ground electrodes comprise a continuous strip.

5. The droplet actuator according to claim 1, further comprising having droplet operations electrode arranged oppositely to each one or more ground electrode.

6. A droplet actuator comprising:

a. a top substrate and a bottom substrate separated to form a droplet operations gap, wherein the droplet operations gap is filled with a filler fluid;

b. a sidewall and an opposite sidewall bounding the droplet operations gap, thereby creating a droplet operations channel;

c. an arrangement of one or more ground electrodes along the sidewall and/or the opposite sidewall, wherein the one or more ground electrodes are connected to an electrical ground;

d. an arrangement of droplet operations electrodes on the bottom substrate,

wherein multiple droplet operations may be conducted on one or more droplets in the droplet operations gap while maintaining substantially consistent contact between the one or more droplets and the one or more ground electrodes, thereby permitting completion of the multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

7. The droplet actuator according to claim 6, wherein the sidewall comprises a first rail and the opposite sidewall comprises a second rail, wherein the first rail and second rail are elongated three-dimensional (3D) structures that are arranged in parallel with each other.

8. A method of performing droplet operations on a droplet in a droplet actuator, comprising:

a. providing a droplet actuator comprising;

i. a top substrate and a bottom substrate separated to form a droplet operations gap;

ii. a sidewall and an opposite sidewall bounding the droplet operations gap, thereby creating a droplet operations channel;

iii. an arrangement of droplet operations electrodes on one of the sidewall or bottom substrate; and

iv. an arrangement of one or more ground electrodes along one or more of the side wall and the opposite sidewall, wherein the one or more ground electrodes are connected to an electrical ground;

b. filling the droplet operations gap of the droplet actuator with a filler fluid;

c. providing a droplet in the droplet operations gap;

d. conducting multiple droplet operations on the droplet in the droplet operations gap, wherein the droplet is transported through the filler fluid in the droplet operations gap; and

e. maintaining substantially consistent contact between the droplet and an electrical ground while conducting the multiple droplet operations on the droplet in the droplet operations gap;

wherein the substantially consistent contact between the droplet and the electrical ground permits completion of the multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

9. The method according to claim 8, wherein the droplet operations electrodes are arranged on the sidewall and the one or more ground electrodes are arranged along the opposite sidewall.

10. The method according to claim 8, wherein the droplet operations electrodes are arranged on the bottom substrate and the one or more ground electrodes are arranged along one or both of the sidewall and the opposite sidewall.

11. The method according to claim 8, wherein the sidewall comprises a first rail and the opposite sidewall comprises a second rail, wherein the first rail and second rail are elongated three-dimensional (3D) structures that are arranged in parallel with each other.

12. The method according to claim 8, further comprising offsetting positions of the droplet operations electrodes and the position of the one or more ground electrodes.

13. The method according to claim 8, wherein the one or more ground electrodes comprise a continuous strip.

14. The method according to claim 8, further comprising oppositely arranging each droplet operations electrode to each one or more ground electrode.

15. A method of performing droplet operations on a droplet in a droplet actuator, comprising:

a. providing a droplet actuator comprising;

i. a top substrate and a bottom substrate separated to form a droplet operations gap;

ii. a sidewall and an opposite sidewall bounding the droplet operations gap, thereby creating a droplet operations channel;

iii. an arrangement of droplet operations electrodes on one of the sidewall or bottom substrate; and

iv. an arrangement of one or more ground electrodes along one or more of the side wall and the opposite sidewall, wherein the one or more ground electrodes are connected to an electrical ground;

b. filling the droplet operations gap of the droplet actuator with a filler fluid;

c. providing a droplet in the droplet operations gap;

d. heating the droplet to within twenty degrees Celsius of boiling to produce a heated droplet;

e. conducting multiple droplet operations on the heated droplet in the droplet operations gap, wherein the heated droplet is transported through the filler fluid in the droplet operations gap; and

f. reducing accumulation of electrical charges in the droplet operations gap as the heated droplet is transported through the filler fluid in the droplet operations gap;

wherein the reduced accumulation of electrical charges in the droplet operations gap permits completion of the multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

16. The method according to claim 15, wherein the droplet operations electrodes are arranged on the sidewall and the one or more ground electrodes are arranged along the opposite sidewall.

17. The method according to claim 15, wherein the droplet operations electrodes are arranged on the bottom substrate and the one or more ground electrodes are arranged along one or both of the sidewall and the opposite sidewall.