Ketol-acid reductoisomerase using NADH
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1. A recombinant mutant ketol-acid reductoisomerase enzyme having the amino acid sequence of SEQ ID NO:
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Abstract
Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
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11 Claims
- 1. A recombinant mutant ketol-acid reductoisomerase enzyme having the amino acid sequence of SEQ ID NO:
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