Apparatus and method for separating and concentrating fluids containing multiple components
First Claim
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1. A method for separating at least one component of a multiple component material with a centrifugal force, the method comprising:
- placing a first volume of a whole material into a container with a buoy separation system including a first buoy member spaced apart from a second buoy member and at least one of the first buoy member or the second buoy member fixed to a connection member;
applying a force to the container including the buoy separation system and the first volume of the whole material, wherein applying the force to the container further operates to;
cause a valve to open to allow moving at least a portion of the first volume of the whole material through a passage defined within at least one of the first buoy member or the second buoy member;
move at least a portion of a moveable sealing portion of the valve away from at least one of the first buoy member or the second buoy member during application of the force, wherein the moveable sealing portion moves towards a valve body that extends along the connection member and away from at least one of the first buoy member and the second buoy member to an unsealed position, wherein the sealing portion is angled relative to at least one of the first buoy member and the second buoy member in the unsealed position; and
separate at least a portion of one component of the multiple component material into a volume defined by the buoy separation system and the container after moving at least the portion of the first volume of the whole material through the passage;
ceasing applying the force to allow the valve to close the passage defined within at least one of the first buoy member and the second buoy member; and
withdrawing at least the portion through the connection member.
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Accused Products
Abstract
An apparatus that allows for separating and collecting a fraction of a sample. The apparatus, when used with a centrifuge, allows for the creation of at various fractions in the apparatus. A buoy system that may include a first buoy portion and a second buoy member operably interconnected may be used to form at least three fractions from a sample during a substantially single centrifugation process. Therefore, the separation of various fractions may be substantially quick and efficient. Also selected fractions from the sample can be applied to a patient, either alone or as part of a mixture.
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Citations
18 Claims
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1. A method for separating at least one component of a multiple component material with a centrifugal force, the method comprising:
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placing a first volume of a whole material into a container with a buoy separation system including a first buoy member spaced apart from a second buoy member and at least one of the first buoy member or the second buoy member fixed to a connection member; applying a force to the container including the buoy separation system and the first volume of the whole material, wherein applying the force to the container further operates to; cause a valve to open to allow moving at least a portion of the first volume of the whole material through a passage defined within at least one of the first buoy member or the second buoy member; move at least a portion of a moveable sealing portion of the valve away from at least one of the first buoy member or the second buoy member during application of the force, wherein the moveable sealing portion moves towards a valve body that extends along the connection member and away from at least one of the first buoy member and the second buoy member to an unsealed position, wherein the sealing portion is angled relative to at least one of the first buoy member and the second buoy member in the unsealed position; and separate at least a portion of one component of the multiple component material into a volume defined by the buoy separation system and the container after moving at least the portion of the first volume of the whole material through the passage; ceasing applying the force to allow the valve to close the passage defined within at least one of the first buoy member and the second buoy member; and withdrawing at least the portion through the connection member. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for separating at least one component of a multiple component material with a centrifugal force, the method comprising:
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placing a first volume of a whole material into a container with a buoy separation system including a first buoy member spaced apart from a second buoy member and at least one of the first buoy member or the second buoy member fixed to a connection member; applying a force to the container including the buoy separation system and the first volume of the whole material, wherein applying the force to the container further operates to; cause a valve to open to allow moving at least a portion of the first volume of the whole material through a passage defined within at least one of the first buoy member or the second buoy member; and separate at least a portion of one component of the multiple component material into a volume defined by the buoy separation system and the container after moving at least the portion of the first volume of the whole material through the passage; ceasing applying the force to allow the valve to close the passage defined within at least one of the first buoy member and the second buoy member; and withdrawing at least the portion through the connection member; wherein the sealing portion moves away from at least one of the first buoy member and the second buoy member by overcoming a spring biasing force; wherein overcoming the spring biasing force includes pushing against a substantially planar surface that extends transverse to an axis of a coil spring that provides the spring biasing force.
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9. A method for separating at least one component of a multiple component material with a centrifugal force, the method comprising:
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placing a first volume of a whole material into a container with a buoy separation system including a first buoy member spaced apart from a second buoy member and at least one of the first buoy member or the second buoy member fixed to a connection member; applying a force to the container including the buoy separation system and the first volume of the whole material, wherein applying the force to the container further operates to; cause a valve to open to allow moving at least a portion of the first volume of the whole material through a passage defined within at least one of the first buoy member or the second buoy member; and separate at least a portion of one component of the multiple component material into a volume defined by the buoy separation system and the container after moving at least the portion of the first volume of the whole material through the passage; ceasing applying the force to allow the valve to close the passage defined within at least one of the first buoy member and the second buoy member; and withdrawing at least the portion through the connection member; wherein the sealing portion moves away from at least one of the first buoy member and the second buoy member by overcoming a spring biasing force; wherein overcoming the spring biasing force includes pushing against a plug that pushes against a coil spring that provides the spring biasing force.
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10. A method for separating at least one component of a multiple component material with a centrifugal force, the method comprising:
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placing a first volume of a whole material into a container with a buoy separation system including a first buoy member spaced apart from a second buoy member and at least one of the first buoy member or the second buoy member fixed to a connection member; applying a force to the container including the buoy separation system and the first volume of the whole material, wherein applying the force to the container further operates to cause a valve to open by moving a sealing portion away from one of the first buoy member or the second buoy member by overcoming a spring biasing force of a spring member biased against the other of the first buoy member or the second buoy member, wherein overcoming the spring biasing force occurs with moving at least a portion of the first volume of the whole material through a passage defined within at least one of the first buoy member or the second buoy member; ceasing applying the force to allow the valve to close the passage defined within at least one of the first buoy member and the second buoy member; and withdrawing at least the portion of one component of the multiple component material through the connection member. - View Dependent Claims (11, 12, 13, 14, 15)
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16. A method to assist in separating at least one component of a multiple component material with a centrifugal force, the method comprising:
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positioning a buoy separation system including a volume between a first buoy member spaced apart from a second buoy member and at least one of the first buoy member or the second buoy member fixed to a connection member; positioning a first valve relative to the second buoy member to open by moving a sealing plug member away from the second buoy member by overcoming a spring biasing force of a spring member biased against a support member extending from the second buoy member away from the first buoy member, wherein overcoming the spring biasing force occurs with moving at least a portion of a first volume of the multiple component material through a first passage defined within the second buoy member; configuring the first valve to close and seal the first passage defined within the second buoy member when a force is removed from the first valve from at least a portion of a first volume of the multiple component material; and configuring the connection member to allow for withdrawal of the first volume of the multiple component material from the volume between the first buoy member spaced apart from the second buoy member through the connection member. - View Dependent Claims (17, 18)
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Specification