×

Restriction endonucleases and their uses

  • US 9,243,233 B2
  • Filed: 07/01/2013
  • Issued: 01/26/2016
  • Est. Priority Date: 07/05/2012
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for determining the level of methylation in double stranded DNA, the method comprisingcontacting a first sample of the double stranded DNA with a first restriction endonuclease comprising SEQ ID NO. 4 or a sequence at least 85% identical to SEQ ID NO. 4, having a recognition sequence 5′

  • -TCGA-3′

    , where a methylated cytosine residue in the recognition sequence impairs cleavage,digesting the DNA comprising the recognition sequence 5′

    -TCGA-3′

    in which the cytosine residue is unmethylated;

    determining the amount of undigested DNA or digested DNA by the first restriction endonuclease,contacting a second sample of the double stranded DNA with a second restriction endonuclease; and

    determining the amount of DNA digestion,where the second restriction endonuclease has a recognition sequence 5′

    -TCGA-3′ and

    is not sensitive to methylation of the cytosine residue.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×