Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
First Claim
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1. A composition comprising a reaction mixture containing a modified nucleotide including a gamma phosphate group attached to a molecular and/or atomic tag, a nucleotide polymerizing agent, a primer, a template and a polyphosphate compound containing at least four phosphate groups.
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Abstract
Nucleotide triphosphate probes containing a molecular and/or atomic tag on a γ and/or β phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.
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1 Claim
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1. A composition comprising a reaction mixture containing a modified nucleotide including a gamma phosphate group attached to a molecular and/or atomic tag, a nucleotide polymerizing agent, a primer, a template and a polyphosphate compound containing at least four phosphate groups.
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