Saliva glucose monitoring system
First Claim
1. A glucose sensor for determining a concentration of glucose in a liquid sample, the sensor comprising:
- an insulating or semiconducting substrate;
at least one working electrode, a counter electrode, and a reference electrode, anda sample placement area on a surface of the substrate for containing the liquid sample during a determination of the glucose concentration in the liquid sample;
wherein each of the working electrode(s), counter electrode, and reference electrode comprises a conductive metal layer deposited on the substrate in the sample placement area;
wherein the working electrode is coated in the sample placement area with a plurality of sensor elements;
wherein the sensor elements in the sample placement area are functionalized with a functionalization coating comprising from 2 to 10 layers, each layer comprising a sublayer of chitosan, a sublayer of gold nanoparticles deposited onto the chitosan, and a sublayer of glucose oxidase deposited onto the gold nanoparticles;
wherein the working electrode, counter electrode, and reference electrode are connected to an amperometry circuit;
wherein the sensor measures electron transfer through the glucose oxidase into the working electrode; and
wherein an output voltage of the amperometry circuit correlates with a glucose concentration in the liquid sample deposited in the sample placement area.
1 Assignment
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Accused Products
Abstract
A glucose sensor suitable for measuring glucose levels in human saliva is provided. Systems containing the glucose sensor and methods for making and using the sensor are also provided. The glucose sensor is highly sensitive and can detect glucose levels at least down to 5 ppm. Fabrication of the sensor involves depositing single-walled carbon nanotubes onto the surface of a working electrode in a 3 electrode electrochemical detector and functionalizing the nanotubes by depositing layers of polymers, metallic nanoparticles, and glucose oxidase enzyme onto the nanotubes. The sensor can be used as a disposable, single-use device or as part of an analytical system, such as a microfluidics system, for the analysis of multiple analytes. The sensor enables the diagnosis and monitoring of diabetes to be performed without pain or the need for finger pricks in a home or clinical setting.
8 Citations
18 Claims
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1. A glucose sensor for determining a concentration of glucose in a liquid sample, the sensor comprising:
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an insulating or semiconducting substrate; at least one working electrode, a counter electrode, and a reference electrode, and a sample placement area on a surface of the substrate for containing the liquid sample during a determination of the glucose concentration in the liquid sample; wherein each of the working electrode(s), counter electrode, and reference electrode comprises a conductive metal layer deposited on the substrate in the sample placement area;
wherein the working electrode is coated in the sample placement area with a plurality of sensor elements;
wherein the sensor elements in the sample placement area are functionalized with a functionalization coating comprising from 2 to 10 layers, each layer comprising a sublayer of chitosan, a sublayer of gold nanoparticles deposited onto the chitosan, and a sublayer of glucose oxidase deposited onto the gold nanoparticles;
wherein the working electrode, counter electrode, and reference electrode are connected to an amperometry circuit;
wherein the sensor measures electron transfer through the glucose oxidase into the working electrode; and
wherein an output voltage of the amperometry circuit correlates with a glucose concentration in the liquid sample deposited in the sample placement area.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification