Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges

• US 9,249,070 B2
• Filed: 05/20/2013
• Issued: 02/02/2016
• Est. Priority Date: 07/02/2008
• Status: Active Grant

• Current Assignee: Goodyear Tire & Rubber Company, Danisco USA Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Goodyear Tire & Rubber Company, Danisco USA Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Calabria, Anthony R., Cervin, Marguerite A., Chotani, Gopal K., La Duca, Richard, McAuliffe, Joseph C., Miller, Michael C., Sabo, Timothy A., Sanford, Karl J., Spring, Erin L., Whited, Gregory M.
• Primary Examiner(s): Kosson, Rosanne
• Application Number:
  US13/898,320
• Publication Number:
  US 20140155660A1
• Time in Patent Office: 988 Days
• US Class Current: 1/1
• CPC Class Codes: C07C 11/18 Isoprene
• CPC Class Codes: C12N 15/52 Genes encoding for enzymes or proenzymes
• CPC Class Codes: C12N 15/70 Vectors or expression systems specially adapted for E. coli
• CPC Class Codes: C12N 15/74 Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
• CPC Class Codes: C12N 15/75 for Bacillus
• CPC Class Codes: C12N 15/80 for fungi
• CPC Class Codes: C12N 9/88 Lyases (4.)
• CPC Class Codes: C12P 5/007 containing one or more isoprene units, i.e. terpenes carotenes C12P23/00
• CPC Class Codes: C12P 5/02 acyclic C12P5/007 takes precedence
• CPC Class Codes: C12Y 402/03027 Isoprene synthase (4.2.3.27)
• CPC Class Codes: Y02E 50/30 Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a nonflammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.

Claims

1. A biologically produced composition comprising:

(a) greater than 2 mg of isoprene and

(b) one or more compounds selected from the group consisting of 2-heptanone, 6-methyl-5-hepten-2-one, 2,4,5-trimethylpyridine, 2,3,5-trimethylpyrazine, citronellal, acetaldehyde, methanethiol, methyl acetate, 1-propanol, diacetyl, 2-butanone, 2-methyl-3-buten-2-ol, ethyl acetate, 2-methyl-1-propanol, 3-methyl-1-butanal, 3-methyl-2-butanone, 1-butanol, 2-pentanone, 3-methyl-1-butanol, ethyl isobutyrate, 3-methyl-2-butenal, butyl acetate, 3-methylbutyl acetate, 3-methyl-3-buten-1-yl acetate, (E)-3,7-dimethyl-1,3,6-octatriene, (Z)-3,7-dimethyl-1,3,6-octatriene, and 2,3-cycloheptenolpyridine, wherein the amount of the one of the one or more compounds relative to the amount of the isoprene is between about 0.01% to 105% (w/w).

2. The composition of claim 1, wherein at least a portion of the isoprene is in the gas phase.

3. The composition of claim 1, wherein the amount of one of the one or more compounds relative to the amount of the isoprene is between about 0.01% to about 90% (w/w), between about 0.01% to about 80% (w/w), between about 0.01% to about 50% (w/w), between about 0.01% to about 20% (w/w), between about 0.01% to about 10% (w/w), between about 0.02% to about 50% (w/w), between about 0.05% to about 50% (w/w), between about 0.1% to about 50% (w/w), or between about 0.1% to about 20% (w/w).

4. The composition of claim 3, wherein the amount of one of the one or more compounds relative to the amount of the isoprene is between about 0.01% to about 90% (w/w).

5. The composition of claim 3, wherein the amount of one of the one or more compounds relative to the amount of the isoprene is between about 0.01% to about 80% (w/w).

6. The composition of claim 3, wherein the amount of one of the one or more compounds relative to the amount of the isoprene is between about 0.01% to about 50% (w/w).

7. The composition of claim 3, wherein the amount of one of the one or more compounds relative to the amount of the isoprene is between about 0.01% to about 20% (w/w).

8. The composition of claim 3, wherein the amount of one of the one or more compounds relative to the amount of the isoprene is between about 0.01% to about 10% (w/w).

9. The composition of claim 3, wherein the amount of one of the one or more compounds relative to the amount of the isoprene is between about 0.02% to about 50% (w/w).

10. The composition of claim 3, wherein the amount of one of the one or more compounds relative to the amount of the isoprene is between about 0.05% to about 50% (w/w).

11. The composition of claim 3, wherein the amount of one of the one or more compounds relative to the amount of the isoprene is between about 0.1% to about 50% (w/w).

12. The composition of claim 3, wherein the amount of one of the one or more compounds relative to the amount of the isoprene is between about 0.1% to about 20% (w/w).