Recyclable extractant compositions
First Claim
1. A process comprising:
- (a) providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway, and butanol;
(b) contacting said fermentation medium with an extractant composition comprising an effective amount of antioxidant, whereby at least a portion of the butanol in the fermentation medium partitions into the extractant;
(c) recovering at least a portion of the butanol and extractant composition from the fermentation medium;
(d) recycling the extractant composition recovered in (c) one or more times to the fermentation medium; and
(e) adjusting the effective amount of antioxidant in the extractant composition whereby the rate of oxidation of recycled extractant composition is substantially reduced such that the extractant composition may be recycled as provided in step (d) for at least ten recycles.
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Accused Products
Abstract
Provided herein are processes comprising providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway, and butanol; contacting said fermentation medium with an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, whereby at least a portion of the butanol in the fermentation medium partitions into the extractant; recovering at least a portion of the butanol and extractant composition from the fermentation medium; recycling the extractant composition recovered in (c) one or more times in the fermentation medium; optionally adjusting the effective amount of antioxidant or antioxidant-like compound in the extractant composition, whereby the rate of oxidation or effect of oxidized products in the recycled extractant composition is substantially reduced and/or avoided such that the extractant composition may be recycled.
86 Citations
22 Claims
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1. A process comprising:
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(a) providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway, and butanol; (b) contacting said fermentation medium with an extractant composition comprising an effective amount of antioxidant, whereby at least a portion of the butanol in the fermentation medium partitions into the extractant; (c) recovering at least a portion of the butanol and extractant composition from the fermentation medium; (d) recycling the extractant composition recovered in (c) one or more times to the fermentation medium; and (e) adjusting the effective amount of antioxidant in the extractant composition whereby the rate of oxidation of recycled extractant composition is substantially reduced such that the extractant composition may be recycled as provided in step (d) for at least ten recycles. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A process comprising:
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(a) providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway, and butanol, wherein the fermentable carbon source is provided by processing biomass; (b) contacting said fermentation medium with an extractant composition, whereby the extractant composition does not comprise an effective amount of antioxidant or antioxidant-like compound, and whereby at least a portion of the butanol in the fermentation medium partitions into the extractant; (c) adjusting the amount of antioxidant or antioxidant-like compound in the extractant composition by further processing the biomass with a protease, whereby the protease stimulates the release of amino acids from the biomass and whereby the amino acids provide the extractant composition with an effective amount of antioxidant or antioxidant-like compound; (d) recovering at least a portion of the butanol and extractant composition from the fermentation medium; (e) recycling the extractant composition recovered in (d) one or more times to the fermentation medium; and (f) adjusting the effective amount of antioxidant or antioxidant-like compound in the extractant composition whereby the rate of oxidation of recycled extractant composition is substantially reduced such that the extractant composition may be recycled as provided in step (e) for at least ten recycles. - View Dependent Claims (11, 12)
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13. A process comprising:
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(a) providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway; and
butanol;(b) contacting said fermentation medium with an extractant composition comprising an effective amount of antioxidant, whereby at least a portion of the butanol in the fermentation medium partitions into the extractant; (c) recovering at least a portion of the butanol and extractant composition from the fermentation medium; (d) adding antioxidants to the extractant composition recovered in (c); and (e) recycling the extractant composition of step (d) one or more times in the fermentation medium.
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14. A biphasic composition comprising:
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(a) a first phase comprising fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered biosynthetic pathway for producing a product alcohol, and a product alcohol; and (b) a second phase comprising an extractant composition comprising an effective amount of antioxidant, product alcohol and free fatty acids; wherein the free fatty acids and antioxidant are derived from the fermentable carbon source. - View Dependent Claims (15)
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16. A method for maintaining the oxidative state of an extractant composition during a butanol fermentation process comprising:
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(a) providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway; and
butanol;(b) contacting said fermentation medium with an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, whereby at least a portion of the butanol in the fermentation medium partitions into the extractant; (c) recovering at least a portion of the butanol and extractant composition from the fermentation medium; (d) monitoring one or more of the effective rate, titer, yield of butanol production or the growth of the recombinant microorganism during fermentation; (e) supplementing the extractant composition with antioxidant following step (d) when said monitoring indicates a butanol toxicity to the recombinant microorganism.
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17. A method for optimizing extraction of butanol from butanol fermentation comprising:
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(a) providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway; and
butanol;(b) providing an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, butanol and free fatty acids, wherein the antioxidant is produced by the recombinant microorganism during fermentation; (c) contacting the fermentation medium of (a) with the extractant composition of (b) whereby the butanol partitions into the extractant composition and is distilled from the extractant. - View Dependent Claims (18)
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19. A method for improving the yield of an isobutanol fermentation comprising:
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(a) providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway; and
butanol;(b) providing an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, butanol and free fatty acids, (c) contacting the fermentation medium of (a) with the extractant composition of (b) whereby butanol partitions into the extractant composition; wherein the yield of isobutanol is improved as compared to yield of isobutanol in the absence of the antioxidant.
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20. A method for improving the titer of isobutanol produced during fermentation comprising:
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(a) providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway; and
butanol;(b) providing an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, butanol and free fatty acids, (c) contacting the fermentation medium of (a) with the extractant composition of (b) whereby butanol partitions into the extractant composition; wherein the titer of isobutanol production is improved as compared to titer of isobutanol production in the absence of the antioxidant.
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21. A method for improving the rate of an isobutanol production during fermentation comprising:
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(a) providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway; and
butanol;(b) providing an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, butanol and free fatty acids, (c) contacting the fermentation medium of (a) with the extractant composition of (b) whereby the butanol partitions into the extractant composition, wherein the rate of isobutanol production is improved as compared to rate of isobutanol production in the absence of the antioxidant.
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22. A process comprising:
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(a) providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway, and butanol; (b) contacting said fermentation medium with an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, whereby at least a portion of the butanol in the fermentation medium partitions into the extractant; (c) recovering at least a portion of the butanol and extractant composition from the fermentation medium; (d) recycling the extractant composition recovered in (c) one or more times to the fermentation medium; and (e) adjusting the effective amount of antioxidant or antioxidant-like compound in the extractant composition whereby the effect of oxidation products of recycled extractant composition is substantially reduced such that the extractant composition may be recycled as provided in step (d) for at least ten recycles.
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Specification