Flexible sample container

• US 9,250,176 B2
• Filed: 03/02/2011
• Issued: 02/02/2016
• Est. Priority Date: 03/04/2010
• Status: Active Grant

• Current Assignee: Koninklijke Philips N.V.
• Original Assignee: Koninklijke Philips N.V.
• Inventors: Olesen, Tom
• Primary Examiner(s): Chowdhury, Tarifur
• Assistant Examiner(s): Gray, Sunghee Y
• Application Number:
  US13/582,593
• Publication Number:
  US 20120327404A1
• Time in Patent Office: 1,798 Days
• Field of Search: 356/244, 356/246, 356432-440, 356335-343
• US Class Current: 1/1
• CPC Class Codes: B01L 2300/0832 cylindrical, tube shaped
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/0364 flexible, compressible
• CPC Class Codes: G01N 2021/8557 Special shaping of flow, e.g. using a by-pass line, jet flow, curtain flow
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/85 Investigating moving fluids or granular solids

Abstract

The invention relates to a system for holding a fluid sample, said system comprising a transparent flexible tube for holding said fluid sample, a tube holder for holding said tube, a first flattening element, and second flattening element, wherein said first flattening element and said second flattening element may be moved relative to each other thereby changing said transparent flexible tube from a first state to a second state, where at least a first cross sectional dimension of said tube is smaller in said second state than in said first state.

Claims

1. A system for holding a fluid sample, comprising:

a transparent flexible tube for holding said fluid sample,

a tube holder for holding said tube,

a first flattening element,

a second flattening element,

wherein at least one of said first flattening element and said second flattening element can be moved relative to each other, thereby changing said transparent flexible tube from a first state to a second state, wherein at least a first cross sectional dimension of said tube is smaller in said second state than in said first state,

an optical scanning apparatus comprising an image acquisition device for acquiring images of the fluid sample comprised in the flexible tube at least in the second state, and

a clamp for clamping said tube in order to maintain the fluid sample at a stand still while the image acquisition device acquires images of the fluid sample.

2. The system according to claim 1, wherein said optical scanning apparatus is adapted to calculate a parameter relating to said fluid sample and from said parameter determine an optimal degree of the first cross sectional dimension for said flexible tube based on the calculated parameter.

3. The system according to claim 1, wherein said transparent flexible tube in a first state has an inner diameter less than about 25 mm.

4. The system according to claim 1, wherein said flexible tube comprises an inlet and/or an outlet, and said system preferably further comprising a tube pump adapted to pump fluid into said tube via said inlet.

5. The system according to claim 1, wherein at least one of said flattening elements comprises a transparent region.

6. The system according to claim 5, wherein said transparent region comprises an inner surface and an outer surface.

7. The system according to claim 6, wherein said inner surface is substantially flat.

8. The system according to claim 7, wherein the activation of the flattening element is such that the distance between the inner walls of said flattened tube is less than about 25 mm.

9. The system according to claim 6, wherein said inner surface comprises a guiding groove.

10. The system according to claim 6, wherein said transparent region comprises at least one optical element selected from a lens, a wedge, a polarizer, an aperture, a filter and a grating.

11. The system according to claim 1, wherein the first flattening element is arranged in an opposing fashion to the second flattening element, and the transparent flexible tube is mounted between the first flattening element and the second flattening element, and at least one of the first flattening element and the second flattening element is a transparent optical element.

12. A method for providing a fluid sample to an optical scanning apparatus, comprising:

arranging a transparent flexible tube in a tube holder,

arranging said tube holder in relation to said optical scanning apparatus,

providing fluid to said tube,

moving at least one of a first flattening element and a second flattening element relative to each other thereby changing said transparent flexible tube from a first state to a second state, wherein at least a first cross sectional dimension of said tube is smaller in the second state than in the first state,

acquiring at least one image of the fluid sample in the transparent flexible tube at least in the second state using said optical scanning apparatus, and

clamping the tube to maintain the fluid sample at a stand still while acquiring the at least one image images of the fluid sample.

13. A method for providing a fluid sample to an optical scanning apparatus, comprising:

arranging a transparent flexible tube in a tube holder,

arranging said tube holder in relation to said optical scanning apparatus,

providing fluid to said tube,

moving at least one of a first flattening element and a second flattening element relative to each other thereby changing said transparent flexible tube from a first state to a second state, wherein at least a first cross sectional dimension of said tube is smaller in the second state than in the first state, and acquiring at least one image from the fluid sample in the transparent flexible tube at least in the second state using said optical scanning apparatus,

acquiring at least one image from said optical scanning apparatus,

determining at least one parameter relating to said fluid sample from said at least one image,

determining a new tube thickness from said at least one parameter, and

moving at least one of said first flattening element and said second flattening element relative to each other thereby changing said transparent flexible tube to a third state based on the new tube thickness.

14. The method according to claim 13, wherein said one or more parameters relates to the concentration of particles in said fluid.

15. The method according to claim 13, wherein said optical apparatus is an optical scanning apparatus adapted to scan an image plane through at least a part of said tube to obtain a plurality of images of the sample arranged therein, and/or to acquire at least one image from said fluid sample in said transparent flexible tube in said first state and/or in said second state, wherein said fluid sample is substantially at stand still.