Methods for detecting antibodies
First Claim
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1. A method for detecting an antibody which is not complexed to an epitope of a target protein, said antibody being in a biological sample obtained from a subject which is suspected of containing such antibody, the method comprising:
- a) synthesizing one or more peptides having a length of about 5-15 amino acids comprising a mimetope recognized by the antibody;
wherein the one or more peptides are attached directly on a solid support and wherein said mimetope comprises a linear sequence of amino acids which is different than a linear sequence of amino acids in said epitope of the target protein recognized by said antibody;
b) contacting the peptide with said biological sample obtained from said subject;
c) allowing binding of the one or more peptides with the antibody not complexed to an antigen to form an antibody-peptide complex; and
d) detecting the antibody-peptide complex in said biological sample obtained from a subject.
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Abstract
Methods for detection of any antibody utilizing a standardized approach applicable to any antibody which provides highly specific assays specific for individual or multiple antibodies. The methods enable improved pharmacokinetic analysis during development and clinical use of antibody-based therapies as well as determination of diagnostic and/or prognostic factors.
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Citations
28 Claims
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1. A method for detecting an antibody which is not complexed to an epitope of a target protein, said antibody being in a biological sample obtained from a subject which is suspected of containing such antibody, the method comprising:
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a) synthesizing one or more peptides having a length of about 5-15 amino acids comprising a mimetope recognized by the antibody;
wherein the one or more peptides are attached directly on a solid support and wherein said mimetope comprises a linear sequence of amino acids which is different than a linear sequence of amino acids in said epitope of the target protein recognized by said antibody;b) contacting the peptide with said biological sample obtained from said subject; c) allowing binding of the one or more peptides with the antibody not complexed to an antigen to form an antibody-peptide complex; and d) detecting the antibody-peptide complex in said biological sample obtained from a subject. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification