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Methods for detecting antibodies

  • US 9,250,233 B2
  • Filed: 03/27/2009
  • Issued: 02/02/2016
  • Est. Priority Date: 03/27/2008
  • Status: Active Grant
First Claim
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1. A method for detecting an antibody which is not complexed to an epitope of a target protein, said antibody being in a biological sample obtained from a subject which is suspected of containing such antibody, the method comprising:

  • a) synthesizing one or more peptides having a length of about 5-15 amino acids comprising a mimetope recognized by the antibody;

    wherein the one or more peptides are attached directly on a solid support and wherein said mimetope comprises a linear sequence of amino acids which is different than a linear sequence of amino acids in said epitope of the target protein recognized by said antibody;

    b) contacting the peptide with said biological sample obtained from said subject;

    c) allowing binding of the one or more peptides with the antibody not complexed to an antigen to form an antibody-peptide complex; and

    d) detecting the antibody-peptide complex in said biological sample obtained from a subject.

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