Method of producing erythrocytes without feeder cells
First Claim
1. A medium for growth or differentiation of hematopoietic cells comprising SCF at a concentration of about 10 to about 10,000 ng/mL, IL-3 at a concentration of about 0.01 to about 500 ng/mL, and EPO at a concentration of about 0.1 to about 10 IU/mL,wherein said medium comprises insulin-like growth factor 1 (IGF-1) at a concentration of about 1 to about 1000 ng/mL,wherein said composition does not comprise one or more of Flt-3L, IL-11, thrombopoietin (Tpo), homeobox-B4 (HoxB4), or methylcellulose,wherein said SCF, IL-3 and Epo are not comprised within an undefined component of said medium, andwherein said medium further comprises lipids at a concentration of about 1 to about 1000 μ
- g/mL, wherein said lipids comprise a mixture of protein and cholesterol.
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Abstract
Provided herein are methods of producing erythrocytes from hematopoietic cells, particularly hematopoietic cells from placental perfusate in combination with hematopoietic cells from umbilical cord blood, wherein the method results in accelerated expansion and differentiation of the hematopoietic cells to more efficiently produce administrable erythrocytes. Further provided herein is a bioreactor in which hematopoietic cell expansion and differentiation takes place.
216 Citations
12 Claims
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1. A medium for growth or differentiation of hematopoietic cells comprising SCF at a concentration of about 10 to about 10,000 ng/mL, IL-3 at a concentration of about 0.01 to about 500 ng/mL, and EPO at a concentration of about 0.1 to about 10 IU/mL,
wherein said medium comprises insulin-like growth factor 1 (IGF-1) at a concentration of about 1 to about 1000 ng/mL, wherein said composition does not comprise one or more of Flt-3L, IL-11, thrombopoietin (Tpo), homeobox-B4 (HoxB4), or methylcellulose, wherein said SCF, IL-3 and Epo are not comprised within an undefined component of said medium, and wherein said medium further comprises lipids at a concentration of about 1 to about 1000 μ - g/mL, wherein said lipids comprise a mixture of protein and cholesterol.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A medium for growth or differentiation of hematopoietic cells comprising SCF at a concentration of about 10 to about 10,000 ng/mL, IL-3 at a concentration of about 5 ng/mL, and EPO at a concentration of about 0.1 to about 10 IU/mL,
wherein said medium comprises insulin-like growth factor 1 (IGF-1) at a concentration of about 1 to about 1000 ng/mL, wherein said composition does not comprise one or more of Flt-3L, IL-11, thrombopoietin (Tpo) homeobox-B4 (HoxB4) or methycellulose, and wherein said SCF, IL-3 and Epo are not comprised within an undefined component of said medium.
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12. A medium for growth or differentiation of hematopoietic cells comprising SCF at a concentration of about 10 to about 10,000 ng/mL, IL-3 at a concentration of about 0.01to about 500 ng/mL, and EPO at a concentration of about 2 to about 3 IU/mL,
wherein said medium comprises insulin-like growth factor 1 (IGF-1) at a concentration of about 1 to about 1000 ng/mL, wherein said composition does not comprise one or more of Flt-3L, IL-11, thrombopoietin (Tpo), homeobox-B4), or methycellulose, and wherein said SCF, IL-3 and Epo are not comprised within an undefined component of said medium.
Specification