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Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples

  • US 9,259,734 B2
  • Filed: 03/09/2012
  • Issued: 02/16/2016
  • Est. Priority Date: 07/13/2007
  • Status: Active Grant
First Claim
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1. An apparatus configured to extract and amplify nucleic acid from a plurality of nucleic-acid containing samples, comprising:

  • a rack comprising a plurality of lanes, each lane of the plurality of lanes comprisinga first location configured to receive one of the plurality of samples, anda second location configured to receive a reagent holder;

    an integrated separator and heater comprisinga magnetic separator configured to move adjacent to one or more of the second locations of the rack; and

    a heater assembly comprising a plurality of independently controllable heater units, each heater unit shaped and sized to accept a single process chamber of a reagent holder, each heater unit configured to apply heat independently of any other heater unit in the heater assembly;

    a liquid dispenser movable from a first position above one of the first locations to a second position above one of the second locations; and

    a bay comprising at least one heat source and configured to receive a microfluidic cartridge comprising a plurality of lanes, the bay configured to interact with the cartridge to separately amplify nucleic acid extracted from the plurality of samples by applying heat to each lane individually.

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