Capture and elution of bio-analytes via beads that are used to disrupt specimens

• US 9,260,475 B2
• Filed: 06/24/2010
• Issued: 02/16/2016
• Est. Priority Date: 06/26/2009
• Status: Active Grant

• Current Assignee: Claremont Biosolutions, LLC
• Original Assignee: Claremont Biosolutions, LLC
• Inventors: Irvine, Bruce, Doebler, Robert W., Erwin, Barbara, Talbot, Ryan P., Niemz, Angelika Brigitte
• Primary Examiner(s): BROWN, MELANIE YU
• Application Number:
  US12/823,081
• Publication Number:
  US 20100331522A1
• Time in Patent Office: 2,063 Days
• US Class Current: 1/1
• CPC Class Codes: C07H 1/06 Separation; Purification
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes
• CPC Class Codes: C12N 1/066 by physical methods
• CPC Class Codes: G01N 2035/00524 Mixing by agitating sample carrier
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form

Abstract

Lysing may include agitating a specimen in a chamber along with a medium that includes a particulate lysing material that has an affinity for a biological material. Lysing material may include beads or other material which may be coated that facilitates binding. The medium may include a fluid with a high salt or low pH level. The biological material may be eluted by lowering a concentration of salt or increasing a pH level. Lysing materials with two or more different affinities may be employed. Heating may be used. Lysing may be performed in a flow through apparatus.

Claims

1. A system to lyse specimens and isolate specific biological materials from the lysed specimens, the system comprising:

a chamber to receive a specimen containing a biological material to be isolated therefrom, the chamber comprising;

a first opening to provide fluid communication with the chamber and to receive said specimen,

a second opening to provide fluid communication with the chamber from an exterior thereof and to provide an exit to the chamber for said specimen, and

at least a first filter positioned adjacent said second opening in a flow path through the chamber, the first filter having a plurality of apertures sized to substantially pass the specimen to be lysed and material that has been lysed and to substantially block particulate lysing material;

a medium that includes the particulate lysing material and a fluid, the particulate lysing material having an affinity for the biological material in the presence of the fluid and the fluid having a physical characteristic that induces binding of the biological material to the particulate lysing material, the affinity sufficient to bind at least some of the biological material to at least some of the particulate lysing material in the presence of the fluid; and

an agitator selectively operable to agitate the specimen in the chamber along with the medium that includes the particulate lysing material and the fluid to mechanically lyse the specimen and concurrently bind at least some of the biological material to at least some of the particulate lysing material.

2. The system of claim 1 wherein the particulate lysing material includes a plurality of beads.

3. The system of claim 1 wherein the particulate lysing material includes at least one of:

a plurality of ceramic beads, a plurality of glass beads, a plurality of zirconium beads, a plurality of silica beads, a plurality of sand, or a plurality of beads with a metal core coated by a material that facilitates binding of the biological material.

4. The system of claim 1 wherein the particulate lysing material has diameters or smallest lateral dimensions in the range of approximately 10 microns to approximately 600 microns.

5. The system of claim 1 wherein the fluid has a high salt concentration.

6. The system of claim 5 wherein the high salt concentration is at least about 200 millimolar.

7. The system of claim 1 wherein the fluid has a pH from about 5.5 to about 3.5.

8. The system of claim 1 wherein the fluid has a pH from about 5.5 to no lower than about 4.

9. The system of claim 1 wherein the medium in the chamber is free of any chemical lysing agents.

10. The system of claim 1 wherein the agitator includes at least one motor and an arm that holds the chamber, the arm coupled to the motor to be oscillatingly driven thereby.

11. The system of claim 1 wherein the agitator includes at least one motor sealingly engaged in a third opening in the chamber and an impeller that are positionable at least partially in the chamber, the impeller coupled to the motor to be rotationally driven thereby.

12. The system of claim 1 wherein the biological material includes at least one of DNA material, a protein or polypeptide material, or another cell component material.

13. The system of claim 1, further comprising:

a chamber or container for receipt of unbound biological material.