Compositions and methods for producing isoprene
First Claim
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1. A method of producing isoprene, the method comprising:
- (a) culturing recombinant cells under suitable culture conditions for the production of isoprene, wherein the cells comprise nucleic acids encoding(i) a heterologous isoprene synthase polypeptide,(ii) an isopentenyl-diphosphate delta-isomerase (DI) polypeptide, and(iii) at least one of a 1-Deoxyxylulose-5-phosphate synthase (DXS)polypeptide and/or one or more mevalonate (MVA) pathway polypeptides and wherein the cells(1) produce greater than about 400 nmole/gwcm/hr of isoprene,(2) convert more than about 0.002 molar percent of the carbon that the cells consume from a cell culture medium into isoprene,(3) have an average volumetric productivity of isoprene greaterthan about 0.1mg/Lbroth/hr of isoprene, or(4) produce at least 0.0037 grams of isoprene per gram of dry cell mass (gdcm); and
(b) producing isoprene.
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Abstract
The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
64 Citations
33 Claims
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1. A method of producing isoprene, the method comprising:
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(a) culturing recombinant cells under suitable culture conditions for the production of isoprene, wherein the cells comprise nucleic acids encoding (i) a heterologous isoprene synthase polypeptide, (ii) an isopentenyl-diphosphate delta-isomerase (DI) polypeptide, and (iii) at least one of a 1-Deoxyxylulose-5-phosphate synthase (DXS)polypeptide and/or one or more mevalonate (MVA) pathway polypeptides and wherein the cells (1) produce greater than about 400 nmole/gwcm/hr of isoprene, (2) convert more than about 0.002 molar percent of the carbon that the cells consume from a cell culture medium into isoprene, (3) have an average volumetric productivity of isoprene greaterthan about 0.1mg/Lbroth/hr of isoprene, or (4) produce at least 0.0037 grams of isoprene per gram of dry cell mass (gdcm); and (b) producing isoprene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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Specification