Flow cytometer

• US 9,261,452 B2
• Filed: 12/23/2013
• Issued: 02/16/2016
• Est. Priority Date: 12/23/2013
• Status: Active Grant

• Current Assignee: Xerox Corporation (Xerox Holdings Corp.)
• Original Assignee: Palo Alto Research Center, Inc. (Xerox Holdings Corp.)
• Inventors: Martini, Joerg, Johnson, Noble M., Recht, Michael I., Johnson, David M., Curley, Tim, Kiesel, Peter, Sheridan, Martin
• Primary Examiner(s): Chowdhury, Tarifur
• Assistant Examiner(s): AKANBI, ISIAKA O
• Application Number:
  US14/139,246
• Publication Number:
  US 20150177119A1
• Time in Patent Office: 785 Days
• Field of Search: 356/440, 356/344, 356/436, 356/446, 356/318, 356/369
• US Class Current: 1/1
• CPC Class Codes: G01N 15/1436 the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/1447 Spatial selection

Abstract

Embodiments are directed to a host structure that includes a waveguide configured to deliver measurement light to a compartment at least partially within the host structure. The compartment is configured to reversibly engage a fluidic optical cartridge. The host structure also includes a detector configured to receive and process output light emanating from the fluidic optical cartridge as well as electronics to process signals from the detector.

Claims

1. A host structure comprising:

a compartment disposed at least partially within the host structure, the compartment being configured to reversibly engage with a fluidic optical cartridge, the compartment including an interlock feature configured to reversibly engage the fluidic optical cartridge, wherein the fluidic optical cartridge comprises;

a fluidic structure that includes a channel through which objects can travel along respective paths during operation of the apparatus, the channel having at least one transparent wall; and

at least one optical component configured to provide measurement light to the objects traveling through the channel, the output light emanating from the objects in response to the measurement light, the host structure further comprising a source of the measurement light;

a waveguide configured to deliver measurement light to the compartment;

a detector configured to receive output light emanating from the fluidic optical cartridge and to generate one or more electrical signals in response to the output light; and

electronics to process the electrical signals from the detector.

2. A host structure according to claim 1, further comprising a handle.

3. A host structure according to claim 1, wherein the at least one optical component comprises a light-redirecting element that can redirect the measurement light from the host structure to the transparent channel of the fluidic structure.

4. A host structure according to claim 3, wherein the light-redirecting element comprises a lens, a lens array, a microlens array, a mirror, or a micromirror array.

5. A host structure according to claim 1, further comprising an analyzer configured to analyze the electrical signals from the detector to identify characteristics of the objects.

6. A host structure according to claim 1, wherein the electronics are configured to output data over a wireless network.

7. A host structure according to claim 1, further comprising a display.

8. A host structure according to claim 1, wherein the light source comprises a laser, a laser diode, a light-emitting diode, a superluminescent diode, a diode-pumped solid state laser, a frequency-doubled laser, a frequency-tripled laser, or a frequency-quadrupled laser.

9. A host structure according to claim 1, further comprising at least one filter assembly configured to provide encoding components to the measurement light emerging from the waveguide.

10. A host structure according to claim 1, further comprising at least one filter assembly configured to provide decoding components to the output light from the compartment.

11. A host structure according to claim 1, wherein the interlock feature comprises an interlock tab.

12. A host structure according to claim 1, wherein a button shaft mechanically or electromechanically connects a button on the host structure with the fluidic optical cartridge.

13. A host structure according to claim 12, wherein the button shaft is configured to allow the optical fluidic cartridged to measure properties of a fluidic sample.

14. A host structure according to claim 1, further comprising a button shaft configured to eject the fluidic optical cartridge from the compartment.

15. A host structure according to claim 1, wherein the fluidic optical cartridge is configured so that when the fluidic optical cartridge is engaged in the compartment a seal is made with the host structure.

16. A flow cytometer comprising a host structure according to claim 1, capable of analyzing objects in fluids.

17. A flow cytometer according to claim 16, wherein the weight of the flow cytometer is 1.0 kg or less.

18. A flow cytometer according to claim 16, wherein the volume of the flow cytometer is a component of the disclosed flow cytometer is 2 L or less.

19. A method comprising:

operating an interlock feature of a compartment of a host structure, operation of the interlock feature reversibly engaging a fluidic optical cartridge in the compartment of the host structure, wherein the host structure comprises;

a waveguide configured to deliver measurement light to a compartment at least partially within the host structure, the compartment being configured to reversibly engage a fluidic optical cartridge;

at least one detector configured to receive output light emanating from the compartment and to generate one or more electrical signals in response to the output light;

electronics configured to process the electrical signals,

wherein the fluidic optical cartridge comprises;

a fluidic structure that includes a channel through which objects can travel along respective paths during operation of the apparatus, the channel having a transparent wall; and

at least one optical component configured to provide the measurement light to the objects traveling through the channel;

drawing analyte fluid into the fluidic structure;

measuring characteristic properties of the objects in the fluid based on the electrical signals; and

dispensing the analyte fluid from the fluidic structure.

20. A method according to claim 19, wherein the at least one optical component comprises a light-redirecting element that can redirect the measurement light from the host structure to the transparent channel of the fluidic structure.

21. A method according to claim 20, wherein the light-redirecting element comprises a lens, a lens array, a microlens array, a mirror, or a micromirror array.

22. A method according to claim 20 comprising using an enzyme-reactive latent fluorophore attached to a support.

23. A method according to claim 20 comprising the use of absorption-encoded microbeads.

24. A method according to claim 19, further comprising ejecting the fluidic optical cartridge from the host structure.

25. A method according to claim 19, further comprising outputting the characteristic properties of the objects to a display on the host structure.

26. A method according to claim 19, further comprising outputting the characteristic properties of the objects to an analyzer.

27. A method according to claim 26, wherein the outputting is over a wireless network.