Biochemical detection unit and biochemical device having the same

• US 9,261,499 B2
• Filed: 03/10/2011
• Issued: 02/16/2016
• Est. Priority Date: 03/10/2010
• Status: Active Grant

• Current Assignee: Crystalvue Medical Corporation
• Original Assignee: Crystalvue Medical Corporation
• Inventors: Chou, Chung-Cheng, Wang, William
• Primary Examiner(s): Prakash, Gautam
• Application Number:
  US13/045,218
• Publication Number:
  US 20120231531A1
• Time in Patent Office: 1,804 Days
• Field of Search: 435/287.2
• US Class Current: 1/1
• CPC Class Codes: G01N 27/126 comprising organic polymers
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms

Abstract

A biochemical detection unit for detecting a sample and a biochemical device having the biochemical detection unit and a releasing unit are provided. The biochemical detection unit includes a photoconductor plate, a receptor, and a resistance sensing component. The receptor specifically binds to the sample so that the illumination projected on the photoconductor plate will change to vary the resistance value of the photoconductor of the photoconductor plate.

Claims

1. A biochemical device, including:

a biochemical detection unit for detecting a sample having at least one epitope for reacting with the biochemical detection unit, including;

a photoconductor plate;

at least one receptor disposed on the photoconductor plate by a bond, the receptor comprising an affinity site for binding specifically to the sample, being variable in conformation and able to produce a conformational change in response to binding to the sample, wherein the conformational change weakens the bond between the receptor and the photoconductor plate and results in the receptor being separated from the photoconductor plate when bound to the sample;

a resistance sensing component, electrically coupled to the photoconductor plate, for sensing a variation in resistance value of the photoconductor plate, wherein the separation of the receptor from the photoconductor plate causes the variation in resistance value of the photoconductor plate;

a light source for illuminating the receptor and the photoconductor, wherein the sample bound to the affinity site of the receptor blocks light from the light source to the photoconductor plate and causes the variation in resistance value of the photoconductor plate; and

a primary channel for the sample entering first and at least one sub-channel for the sample entering later on;

wherein the at least one sub-channel communicates with the primary channel through an opening, wherein the photoconductor plate is disposed in the primary channel or the sub-channel, at least one portion of the sample from the primary channel enters at least one sub-channel towards the photoconductor plate; and

a sampling unit, disposed by the opening and electrically connected to the resistance sensing component, for selectively allowing the sample to enter the sub-channel upon concentration of the sample, wherein the resistance sensing component outputs a control signal to the sampling unit for the sampling unit closing the opening when the resistance sensing component senses the resistance value varying over a default value.

2. The biochemical device of claim 1, wherein the sampling unit includes a semi-transparent membrane.

3. The biochemical device of claim 1, wherein the sampling unit includes a valve, the valve is electrically connected to the resistance sensing component, the resistance sensing component outputs a control signal to the valve for the valve closing the opening when the resistance sensing component senses the resistance value varying over a default value.

4. The biochemical device of claim 1 further comprising

a controllable agent importing device disposed externally to the biochemical detection unit, comprising at least one containing space and releasing at least one agent to react with the receptor bound to the sample to cause the variation in resistance value;

wherein the biochemical device in sequence has the affinity site binding to the sample and the controllable agent importing device releasing the at least one agent;

wherein an opening time and a sequence of opening of the controllable agent importing device are controlled so as to release the at least one agent from the at least one containing space after the sample bound to the affinity site.

5. The biochemical device of claim 4, wherein the at least one agent includes at least one affinity agent having a binding portion for binding to the affinity site being already bound to the sample and a reaction portion, wherein the releasing unit further releases at least one luminescence-reactive agent, a fluorescence having a specific wavelength range is produced and emitted after the luminescence-reactive agent reacting with the reaction portion, the fluorescence stimulates the photoconductor plate and causes the variation in resistance value of the photoconductor plate.

6. The biochemical device of claim 5, wherein the specific wavelength range is selected from 620˜

750 nm, 495˜

570 nm, and 358˜

461 nm.

7. The biochemical device of claim 4, wherein the at least one agent includes at least one affinity agent having a binding portion for binding to the affinity site being already bound to the sample and a reaction portion for self-emitting a light having a specific wavelength range, the light stimulates the photoconductor plate and causes the variation in resistance value of the photoconductor plate.

8. The biochemical device of claim 7, wherein the specific wavelength range is selected from 620˜

750 nm, 495˜

570 nm, and 358˜

461 nm.

9. The biochemical device of claim 4 wherein the at least one agent includes at least one affinity agent having a binding portion for binding to the affinity site being already bound to the sample and a fluorescent reaction portion for emitting a fluorescence having a specific wavelength range after being illuminated by light from the light source, the fluorescence stimulates the photoconductor plate and causes the variation in resistance value of the photoconductor plate.

10. The biochemical device of claim 9, wherein the specific wavelength range is selected from 620˜

750 nm, 495˜

570 nm, and 358˜

461 nm.

11. The biochemical device of claim 4, wherein the at least one agent includes a first affinity agent and a second affinity agent, the first affinity agent comprises a first binding portion for binding specifically to the affinity site being already bound to the sample and a first fluorescent reaction portion, the second affinity agent comprises a second binding portion for binding specifically to a blocking site of the receptor and a second fluorescent reaction portion, the second fluorescent reaction portion emits a fluorescence having a first wavelength range after being illuminated by light from the light source, the fluorescence having the first wavelength range excites the first fluorescent reaction portion to produce a fluorescence having a second wavelength range to further stimulate the photoconductor plate and cause the variation in resistance value of the photoconductor plate.

12. The biochemical device of claim 11, wherein the first wavelength range and the second wavelength range are selected from 620˜

750 nm, 495˜

570 nm, and 358˜

461 nm, and the first wavelength range does not overlap the second wavelength range.

13. The biochemical device of claim 4, wherein the at least one agent includes a first affinity agent, a second affinity agent, and at least one luminescence-reactive agent, the first affinity agent comprises a first binding portion for binding specifically to the affinity site being already bound to the sample and a first fluorescent reaction portion, the second affinity agent comprises a second binding portion for binding specifically to a blocking site of the receptor and a second fluorescent reaction portion, a fluorescence having a first wavelength range is produced and emitted after the luminescence-reactive agent reacting with the second fluorescent reaction portion, the first fluorescence having the first wavelength range excites the first fluorescent reaction portion to produce a fluorescence having a second wavelength range to further stimulate the photoconductor plate and causes the variation in resistance value of the photoconductor plate.

14. The biochemical device of claim 13, wherein the first wavelength range and the second wavelength range are selected from 620˜

750 nm, 495˜

570 nm, and 358˜

461 nm, and the first wavelength range does not overlap the second wavelength range.