Methods for modulating cell function

• US 9,265,969 B2
• Filed: 12/10/2012
• Issued: 02/23/2016
• Est. Priority Date: 12/21/2011
• Status: Active Grant

• Current Assignee: Cardiac Pacemakers Incorporated (Boston Scientific Corporation)
• Original Assignee: Cardiac Pacemakers Incorporated (Boston Scientific Corporation)
• Inventors: Shuros, Allan C., Stolen, Craig
• Primary Examiner(s): Thomson, William
• Assistant Examiner(s): Downey, John R
• Application Number:
  US13/709,744
• Publication Number:
  US 20130165844A1
• Time in Patent Office: 1,170 Days
• Field of Search: 607/92
• US Class Current: 1/1
• CPC Class Codes: A61N 5/062 Photodynamic therapy, i.e. excitation of an agent
• CPC Class Codes: A61N 5/0622 Optical stimulation for exciting neural tissue

Abstract

Methods for modulating nerve function are disclosed. An example method for modulating nerve function may include providing a transgene including a neuron-specific promoter and a gene encoding a light-sensitive protein, delivering the transgene to a body tissue including one or more target neurons, implanting a light source adjacent to the cell bodies of the one or more target neurons, and emitting light from the light source. Light may be exposed to the cell bodies of the one or more target neurons and may cause a conformational change in the light-sensitive protein.

Claims

1. A method for treating hypertension, the method comprising:

delivering a viral vector to a body tissue of a patient, wherein the body tissue includes one or more neurons;

wherein the viral vector includes a transgene, the transgene including a neuron-specific promoter and a gene encoding a nerve modulation protein, the transgene also including a second gene encoding an enzyme that converts a non-toxic form of a therapeutic agent to a toxic form;

infecting the neurons with the viral vector;

implanting a light source adjacent to the cell bodies of the one or more neurons; and

emitting light from the light source;

monitoring the patient for a change in blood pressure; and

if a change in blood pressure is observed, administering the non-toxic form of the therapeutic agent to the body tissue;

wherein the enzyme converts the non-toxic form of the therapeutic agent to the toxic form.

2. The method of claim 1, wherein emitting light from the light source causes a structural change in the nerve modulation protein and causes hyperpolarization of the neurons.

3. The method of claim 1, wherein the nerve modulation protein includes a light-sensitive ion channel.

4. The method of claim 1, wherein the nerve modulation protein includes a light-sensitive chloride ion pump.

5. The method of claim 1, wherein the viral vector includes adenovirus or an adeno-assisted virus.

6. The method of claim 1, wherein implanting a light source adjacent to the cell bodies of the one or more neurons includes implanting the light source in an epidural space of a patient.

7. The method of claim 1, wherein emitting light from the light source inactivates the one or more neurons.

8. The method of claim 1, wherein the light source includes a blood pressure sensor, wherein the method includes measuring blood pressure with the blood pressure sensor, and wherein the amount of light emitted from the light source is altered based on the measured blood pressure.

9. The method of claim 1, wherein the light source includes a body position sensor, wherein the method includes measuring body position with the body position sensor, and wherein the amount of light emitted from the light source is altered based on the measured body position.

10. The method of claim 1, wherein emitting light from the light source includes emitting light for one or more discrete time periods.

11. A method for treating hypertension, the method comprising:

providing a viral vector carrying a transgene, the transgene including a light-sensitive promoter that drives expression of a first gene encoding a nerve modulation protein and a second gene encoding an enzyme that converts a non-toxic form of a toxin to a toxic form of the toxin;

delivering the viral vector to a body tissue of a patient, wherein the body tissue includes one or more neurons;

infecting the neurons with the viral vector;

implanting a light source adjacent to the cell bodies of the one or more neurons;

emitting light of a first wavelength from the light source, wherein light of the first wavelength is exposed to the cell bodies of the one or more neurons and drives expression of the first gene and the second gene;

emitting light of a second wavelength from the light source;

measuring blood pressure in the patient; and

if a change in blood pressure is observed, administering the non-toxic form of the toxin to the body tissue;

wherein the enzyme converts the non-toxic form of the toxin to the toxic form and kills the body tissue.