Systems and methods for utilizing and recovering chitosan to process biological material

• US 9,266,973 B2
• Filed: 03/15/2013
• Issued: 02/23/2016
• Est. Priority Date: 03/15/2013
• Status: Expired due to Fees

• Current Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Original Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Inventors: Fleischer, Daniel
• Primary Examiner(s): Carlson, Karen Cochrane
• Assistant Examiner(s): Tichy, Jennifer M. H.
• Application Number:
  US13/839,647
• Publication Number:
  US 20140273176A1
• Time in Patent Office: 1,075 Days
• Field of Search: 435/261, 435/308.1, 536/20
• US Class Current: 1/1
• CPC Class Codes: C08B 37/0003 General processes for their isolation or fractionation, e.g. purification or extraction from biomass
• CPC Class Codes: C08B 37/003 Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
• CPC Class Codes: C12M 47/02 Separating microorganisms from the culture medium; Concentration of biomass separating microorganisms from their culture media C12N1/02
• CPC Class Codes: C12P 19/04 Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• CPC Class Codes: C12P 19/26 Preparation of nitrogen-containing carbohydrates

Abstract

Systems and methods for processing biological material utilizing chitosan are provided herein. Methods may include the steps of reducing the pH of an amount of culture to about 5 to 7, incorporating an amount of a chitosan solution into the culture to form a mixture, wherein chitosan in the chitosan solution binds to the biological material; increasing the pH of the culture to about 7 to 10, to flocculate biological material in the culture, concentrating the flocculates, reducing the pH of the concentrated flocculates to about 3 to 5, and separating the recovered chitosan solution from the concentrated biological material.

Claims

1. A system adapted to utilize and recover chitosan for processing biological material, comprising:

a culture source that retains culture, the culture including an amount of biological material suspended within a fluid;

a mixer in fluid communication with the culture source and an acid source, the mixer adapted to reduce the pH of an amount of the culture to about 5 to 7, inclusive, by facilitating one or more chemical processes within the culture;

a flocculation apparatus receiving the culture and incorporating an amount of a chitosan solution into the culture to form a mixture, wherein chitosan in the chitosan solution binds to the biological material, the flocculation apparatus adapted to then receive an amount of a base from a base source to increase the pH of the mixture to about 7 to 10, inclusive, to cause the biological material bound to the chitosan to form flocculates that are dispersed in the fluid and subsequently concentrated; and

a separator apparatus to separate concentrated biological material from the concentrated flocculates, the separator apparatus being adapted to receive an amount of an acid to reduce the pH of the concentrated flocculates to about 3 to 5, inclusive, to remove recovered chitosan from the concentrated biological material;

wherein the recovered chitosan and the concentrated biological material are mechanically processed by the separator apparatus to separate the recovered chitosan from the concentrated material; and

wherein the recovered chitosan is incorporated with the chitosan solution.

2. The system according to claim 1, wherein the biological material includes microalgae cells of the genus Nannochloropsis.

3. The system according to claim 1, wherein the one or more chemical processes include at least one of:

sparging an amount of carbon dioxide into the culture; and

combining the amount of the culture with an amount of an acid.

4. The system according to claim 1, wherein the amount of chitosan solution incorporated includes about 0.5 to 200 milligrams of chitosan per liter of the culture.

5. The system according to claim 1, wherein the mixer includes at least one static mixer.

6. The system according to claim 1, wherein the base includes at least one of calcium oxide, calcium hydroxide, sodium hydroxide, magnesium hydroxide, and ammonium hydroxide.

7. The system according to claim 6, wherein the amount of the base includes a sufficient amount to raise the pH of the mixture a sufficient amount to cause substantially all of the suspended biological material to form flocculates.

8. The system according to claim 1, wherein the flocculation apparatus includes a dissolved air flotation device adapted to create micro-bubbles that associate with the flocculates and carry the flocculates to a surface of the fluid.

9. The system according to claim 1, wherein the separator apparatus includes at least one of a filter or series of filters, a decanter, centrifuge, and combinations thereof.

10. The system according to claim 1, wherein the mixer and the flocculation apparatus are combined and the separator apparatus includes a centrifuge.

11. The system according to claim 1, wherein the recovered chitosan solution is returned to a source of the chitosan solution.

12. The system according to claim 1, further comprising:

an additional vessel adapted to receive the concentrated biological material from the separator and combine the concentrated biological material with fresh water such that additional chitosan is removed from the concentrated biological material;

wherein the concentrated biological material combined with fresh water are provided to the separator to extract additional chitosan from the concentrated biological material.

13. The system according to claim 1, further comprising an additional separator apparatus adapted to receive the concentrated biological material from the separator and combine the concentrated biological material with fresh water, wherein the additional separator is adapted to mechanically process the concentrated biological material combined with fresh water to extract additional chitosan from the concentrated biological material.

14. The system according to claim 13, wherein mechanically process includes at least one of filtering, decanting, centrifuging, and combinations thereof.