Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array
First Claim
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1. An electrochemical deblocking solution comprising:
- an organic base including N,N-diisopropylethylamine, a benzoquinone and a hydroquinone;
tetrabutylammonium hexafluorophosphate as an organic salt; and
a solvent including acetonitrile and an alcohol.
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Abstract
There is disclosed an electrochemical deblocking solution for use on an electrode microarray. There is further disclosed a method for electrochemical synthesis on an electrode array using the electrochemical deblocking solution. The solution and method are for removing acid-labile protecting groups for synthesis of oligonucleotides, peptides, small molecules, or polymers on a microarray of electrodes while substantially improving isolation of deblocking to active electrodes. The method comprises applying a voltage or a current to at least one electrode of an array of electrodes. The array of electrodes is covered by the electrochemical deblocking solution.
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Citations
20 Claims
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1. An electrochemical deblocking solution comprising:
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an organic base including N,N-diisopropylethylamine, a benzoquinone and a hydroquinone; tetrabutylammonium hexafluorophosphate as an organic salt; and a solvent including acetonitrile and an alcohol. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. An electrochemical deblocking solution comprising:
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an organic base including a lutidine, a benzoquinone and a hydroquinone; tetraethylammonium p-toluenesulfonate as an organic salt; and a solvent selected from the group consisting of two or more of an alcohol, acetonitrile and dichloromethane. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. An electrochemical deblocking solution for use on an electrode microarray comprising:
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approximately 0.01 to 200 mM of a pyridine derivative or isomer thereof, where the pyridine derivative or isomer thereof is not bound to the electrode microarray; approximately 0.1 to 100 mM 2,5-di-tert-butyl benzoquinone; approximately 0.1 to 100 mM 2,5-di-tert-butyl hydroquinone; approximately 0.1 mM to 5 M tetraethylammonium p-toluenesulfonate; and a solvent having approximately 10% to 99% acetonitrile and approximately 1% to 90% methanol. - View Dependent Claims (20)
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Specification