Nanopores in zero mode waveguides
First Claim
1. An array of zero mode waveguides, each zero mode waveguide comprising an aperture having walls and a base, the base comprising a thin membrane, the array comprising a top solution above the zero mode waveguides extending into each zero mode waveguide, and a bottom solution below the thin membranes comprising the bases of the zero mode waveguides, wherein each zero mode waveguide further comprises a nanopore extending through the thin membrane to the solution below the zero mode waveguide.
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Abstract
Methods, devices, substrates, and systems are disclosed involving arrays of zero-mode waveguides having nanopores extending through the bases that form the bottoms of the zero-mode-waveguides. Electric fields across the nanopores are used to attach single biomolecules such as polymerase enzymes within each zero-mode-waveguide. Electric fields across the nanopores can also be used for the active loading of nucleic acid templates into enzymes attached within the zero mode waveguides.
34 Citations
18 Claims
- 1. An array of zero mode waveguides, each zero mode waveguide comprising an aperture having walls and a base, the base comprising a thin membrane, the array comprising a top solution above the zero mode waveguides extending into each zero mode waveguide, and a bottom solution below the thin membranes comprising the bases of the zero mode waveguides, wherein each zero mode waveguide further comprises a nanopore extending through the thin membrane to the solution below the zero mode waveguide.
Specification