Formulation and delivery of PLGA microspheres
First Claim
1. A method of producing a protein of interest in a cell comprising contacting the cell with a PLGA microsphere particle, said PLGA microsphere particle consisting essentially of PLGA and having no amine-containing polymers or amino acids, said PLGA microsphere particle comprising a modified mRNA greater than 30 nucleotides in length encoding said protein of interest, wherein said modified mRNA consists of a nucleoside modification which is N1-methylpseudouridine in place of uridines, wherein the PLGA microsphere particle has a percent encapsulation efficiency of at least 68.1%, a particle size of at least 40 um and an actual RNA loading of at least 0.31 weight percent.
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Accused Products
Abstract
The present disclosure provides, inter alia, formulation compositions comprising modified nucleic acid molecules which may encode a protein, a protein precursor, or a partially or fully processed form of the protein or a protein precursor. The formulation composition may further include a modified nucleic acid molecule and a delivery agent. The present invention further provides nucleic acids useful for encoding polypeptides capable of modulating a cell'"'"'s function and/or activity.
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Citations
9 Claims
- 1. A method of producing a protein of interest in a cell comprising contacting the cell with a PLGA microsphere particle, said PLGA microsphere particle consisting essentially of PLGA and having no amine-containing polymers or amino acids, said PLGA microsphere particle comprising a modified mRNA greater than 30 nucleotides in length encoding said protein of interest, wherein said modified mRNA consists of a nucleoside modification which is N1-methylpseudouridine in place of uridines, wherein the PLGA microsphere particle has a percent encapsulation efficiency of at least 68.1%, a particle size of at least 40 um and an actual RNA loading of at least 0.31 weight percent.
Specification