Methods and compositions for processes of rapid selection and production of nucleic acid aptamers
First Claim
1. A composition comprising at least one double-stranded nucleic acid molecule, the at least one double-stranded nucleic acid molecule comprising:
- a reporter moiety bound to a fully-annealed first strand of the double- stranded nucleic acid molecule wherein the reporter moiety is capable of generating a signal; and
a signal reducing moiety bound to a second, separate and fully complementary strand of the first nucleic acid strand wherein the signal reducing moiety is capable of reducing the signal produced by the reporter moiety,wherein the first and the second strand of the double-stranded nucleic acid molecule are the same length, and wherein either the first nucleic acid strand or the second nucleic acid strand of the double-stranded nucleic acid molecule is an aptamer and binds in a non-complementary manner to a target agent.
2 Assignments
0 Petitions
Accused Products
Abstract
Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.
-
Citations
17 Claims
-
1. A composition comprising at least one double-stranded nucleic acid molecule, the at least one double-stranded nucleic acid molecule comprising:
-
a reporter moiety bound to a fully-annealed first strand of the double- stranded nucleic acid molecule wherein the reporter moiety is capable of generating a signal; and a signal reducing moiety bound to a second, separate and fully complementary strand of the first nucleic acid strand wherein the signal reducing moiety is capable of reducing the signal produced by the reporter moiety, wherein the first and the second strand of the double-stranded nucleic acid molecule are the same length, and wherein either the first nucleic acid strand or the second nucleic acid strand of the double-stranded nucleic acid molecule is an aptamer and binds in a non-complementary manner to a target agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15, 16, 17)
-
- 13. A composition comprising, a quenching agent moiety bound to at least one separate and fully complementary strand of a fully-annealed dsDNA molecule, wherein absorption wavelength of the quenching agent moiety overlaps with emission wavelength of an agent capable of generating a signal, wherein one of the separate and fully complementary strands of the fully-annealed dsDNAmolecule is an aptamer and binds in a non-complementary manner to a non-nucleic acid target agent, and wherein the strands of the dsDNA molecule are the same length.
Specification