Instrument for monitoring polymerase chain reaction of DNA

• US 9,273,353 B2
• Filed: 06/04/2015
• Issued: 03/01/2016
• Est. Priority Date: 05/16/1998
• Status: Expired due to Fees

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Atwood, John G., Cerrone, Anthony L., Lakatos, Edward J., Young, Eugene F., Gambini, Michael R.
• Primary Examiner(s): HOBBS, MICHAEL L
• Application Number:
  US14/730,989
• Publication Number:
  US 20150322488A1
• Time in Patent Office: 271 Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0663 Whole sensors
• CPC Class Codes: B01L 2300/1811 using electromagnetic induction heating
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2545/101 with an internal standard/control
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: G01N 2021/6419 Excitation at two or more wavelengths
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 2021/6463 Optics
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 2201/0245 with insertable-removable part
• CPC Class Codes: G01N 2201/06113 : Coherent sources; lasers
• CPC Class Codes: G01N 2201/0628 : Organic LED [OLED]
• CPC Class Codes: G01N 2201/0635 : Structured illumination, e.g. with grating
• CPC Class Codes: G01N 2201/0636 : Reflectors
• CPC Class Codes: G01N 2201/068 : Optics, miscellaneous

Abstract

An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

Claims

1. An instrument comprising:

an apparatus configured to hold a plurality of spaced-apart reaction regions;

a light source configured to direct an excitation beam toward the apparatus;

a detector configured to receive emission beams from the apparatus;

an excitation beam path disposed from the light source to the apparatus;

an emission beam path disposed from the apparatus to the detector; and

a lens disposed along the excitation beam path and along the emission beam path, the lens configured to direct the excitation beam to more than one of the reaction regions simultaneously.

2. The instrument of claim 1, wherein the light source comprises a laser.

3. The instrument of claim 1, further comprising a dichroic reflector configured to reflect the excitation beam.

4. The instrument of claim 1, wherein the light source is configured to emit at least two excitation beams, each excitation beam characterized by a central wavelength that is different from that of the other excitation beam(s).

5. The instrument of claim 4, further comprising a plurality of dichroic reflectors configured to reflect the at least two excitation beams from the light source.

6. The instrument of claim 1, wherein lens comprises a focusing optic.

7. The instrument of claim 1, wherein the lens is located proximal to the apparatus.

8. The instrument of claim 1, wherein the instrument comprises the plurality of spaced-apart reaction regions and at least some of the reaction regions comprise one or more of a component for DNA replication, a fluorescent dye, or a dye labeled probe.

9. The instrument of claim 1, wherein the instrument comprises the plurality of spaced-apart reaction regions wherein at least two reaction regions each produce an emission beams that simultaneously passes through the lens and onto the detector.

10. The instrument of claim 1, wherein the apparatus comprises a PCR reaction apparatus.

11. The instrument of claim 1, wherein the apparatus comprises a heater to maintain a temperature.

12. An instrument comprising:

an apparatus having a surface configured to hold a plurality of spaced-apart reaction regions;

a light source configured to generate an excitation beam;

a detector configured to detect emission beams from the apparatus;

an excitation beam path disposed from the light source to the surface of the apparatus;

an emission beam path disposed from the surface of the apparatus to the detector; and

a lens disposed along the excitation beam path and the emission beam path, the lens configured to direct the excitation beam to the surface configured to hold a plurality of spaced-apart reaction regions;

wherein said lens is configured to simultaneously illuminate a plurality of spaced-apart reaction regions.

13. The instrument of claim 12, wherein the light source comprises a laser.

14. The instrument of claim 12, further comprising a dichroic reflector configured to reflect the excitation beam.

15. The instrument of claim 12, wherein the light source emits at least two excitation beams, each excitation beam characterized by a central wavelength that is different from that of the other excitation beam(s).

16. The instrument of claim 15, further comprising a plurality of dichroic reflectors configured to reflect light from the light source.

17. The instrument of claim 12, wherein lens comprises a focusing optic.

18. The instrument of claim 12, wherein the apparatus comprises a PCR reaction apparatus.

19. The instrument of claim 12, wherein the apparatus comprises a heater to maintain a temperature.

20. An instrument comprising:

an apparatus having a portion configured to hold a plurality of spaced-apart reaction regions;

a light source configured to direct an excitation beam toward the portion;

a detector configured to receive emission beams from the portion; and

a lens configured to direct the excitation beam to the portion, to illuminate more than one reaction region simultaneously, and to direct emission beams from the portion to the detector to produce data signals.

21. The instrument of claim 20, wherein the light source comprises a laser.

22. The instrument of claim 20, further comprising a dichroic reflector configured to reflect the excitation beam.

23. The instrument of claim 20, wherein the light source emits at least two excitation beams, each excitation beam characterized by a central wavelength that is different from that of the other excitation beam(s).

24. The instrument of claim 23, further comprising a plurality of dichroic reflectors configured to reflect light from the light source.

25. The instrument of claim 20, wherein lens comprises a focusing optic.

26. The instrument of claim 20, wherein the apparatus comprises a PCR reaction apparatus.

27. The instrument of claim 20, wherein the apparatus comprises a heater to maintain a temperature.