Methods for assessing cell culture fluid by impedance spectra
First Claim
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1. A method for assessing a cell culture reaction fluid of a cell culture reaction, comprising the steps of:
- generating a plurality of frequencies with a sensor comprising a sensing region in operational contact with the cell culture reaction fluid and a plurality of tuning circuits outside the fluid;
receiving a signal from the sensor, wherein the signal is representative of impedance spectra of the sensing region in operational contact with the cell culture reaction fluid over a measured spectral frequency range;
analyzing the impedance spectra; and
determining one or more properties of the cell culture reaction fluid based on the analyzed impedance spectra.
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Abstract
Provided herein are techniques for identification of viable and nonviable cells in a cell culture that include measuring a resonance impedance spectral response of at least one resonator in proximity to the cell culture and correlating the measured response to the concentration of viable cells in cell culture and/or the concentration of nonviable cells in cell culture.
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Citations
18 Claims
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1. A method for assessing a cell culture reaction fluid of a cell culture reaction, comprising the steps of:
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generating a plurality of frequencies with a sensor comprising a sensing region in operational contact with the cell culture reaction fluid and a plurality of tuning circuits outside the fluid; receiving a signal from the sensor, wherein the signal is representative of impedance spectra of the sensing region in operational contact with the cell culture reaction fluid over a measured spectral frequency range; analyzing the impedance spectra; and determining one or more properties of the cell culture reaction fluid based on the analyzed impedance spectra. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification