Microfluidic chip comprising several cylinder-piston arrangements

• US 9,278,352 B2
• Filed: 09/27/2011
• Issued: 03/08/2016
• Est. Priority Date: 09/30/2010
• Status: Expired due to Fees

• Current Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Original Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Inventors: Drese, Klaus-Stefan, Latta, Daniel, Ritzi-Lehnert, Marion, Roeser, Tina
• Primary Examiner(s): Alexander, Lyle
• Assistant Examiner(s): Wecker, Jennifer
• Application Number:
  US13/824,817
• Publication Number:
  US 20130209328A1
• Time in Patent Office: 1,624 Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2300/0858 Side walls
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 3/0217 of the plunger pump type medical syringes A61M
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: F16K 2099/0084 Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 99/0001 Microvalves microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006

Abstract

A microfluidic arrangement including a substrate in which a microfluidic structure having several adjacent channels and at least one common supply line, into which the adjacent channels merge, is formed. Each of the adjacent channels form the cylinder of a cylinder-piston arrangement for receiving an associated piston. A method for producing the type of microfluidic-arrangement is also disclosed.

Claims

1. A microfluidic arrangement comprising:

a substrate in which a microfluidic structure is formed, the latter having a plurality of parallel adjacent channels each having an open top side, and at least one common supply line, into which one end of the adjacent channels open, wherein a portion of each of the adjacent channels forms a cylinder section of a cylinder/piston arrangement, with the other end of the adjacent channels forming an aperture sized to receive a piston;

a plurality of elongate pistons each associated with one of the plurality of channels and having a cross sectional area significantly less than the associated channel, wherein the pistons each have a free end which extends through the aperture into the cylinder section of one of the channels;

a cover cooperating with the top side of the substrate and configured to sealingly isolate the parallel adjacent channels from the outside environment;

and a sealing arrangement, which is stationary relative to the substrate, for sealing each of the pistons in an associated cylinder adjacent a second end of the channel;

wherein the pistons are configured to move axially through the sealing arrangement, enabling the free end to move into or out of the cylinder section; and

wherein the sealing arrangement comprises a continuous seal element which spans the plurality of adjacent channels and seals a plurality of the pistons.

2. The microfluidic arrangement as claimed in claim 1, wherein the sealing arrangement comprises a sealing channel, which crosses the plurality of adjacent channels, for receiving the continuous seal element.

3. The microfluidic arrangement as claimed in claim 1, wherein the seal element is applied on an end side of the substrate of the microfluidic arrangement.

4. The microfluidic arrangement as claimed in claim 2, wherein the seal element consists of a polymer.

5. The microfluidic arrangement as claimed in claim 4, wherein the seal element is connected to the substrate of the microfluidic arrangement by injection molding.

6. The microfluidic arrangement as claimed in claim 1, further comprising a common actuation element wherein a plurality of pistons each assigned to one of the adjacent channels are coupled to one another by the common actuation element to enable the coupled pistons to be move in unison.

7. The microfluidic arrangement as claimed in claim 1, wherein the pistons are introduced into the substrate of the microfluidic arrangement through apertures on the substrate adjacent a second end of the channel.

8. The microfluidic arrangement as claimed in claim 6, wherein the common supply line has a sufficient volume to ensure that all the adjacent channels can be filled simultaneously and uniformly when the common actuating element is moved withdrawing the pistons in unison.

9. The microfluidic arrangement as claimed in claim 1, wherein the common supply line is formed by a channel which extends transversely on the top side of the substrate with respect to the adjacent channels and has two access ports.

10. The microfluidic arrangement as claimed in claim 9, wherein at least one of the access ports can be sealed in a gas-tight fashion.

11. A method for producing a microfluidic arrangement as claimed in claim 1, wherein the microfluidic arrangement is produced by an injection molding method, the pistons being placed in an injection mold and at least one plastic being injected around them.

12. The method as claimed in claim 11, wherein the pistons are placed in the injection mold after the injection molding of the substrate or with the completed substrate, and a polymer seal element is then injected around them.

13. The microfluidic arrangement as claimed in claim 1, wherein the cover comprises a film layer.

15. The microfluidic arrangement as claimed in claim 1, wherein the plurality of elongate pistons each has a common uniform diameter.

16. The microfluidic arrangement as claimed in claim 1, wherein the diameter of the pistons is in the range of a millimeter or less.

17. The microfluidic arrangement as claimed in claim 1, wherein the pistons each have a free end which an end which outwardly extends from a side of the substrate.

18. The microfluidic arrangement as claimed in claim 1, wherein the pistons each have a round cylindrical wire-like shape sized to freely move within the channel section without touching the side walls.

14. A microfluidic arrangement comprising:

a substrate in which a microfluidic structure is formed, the latter having a plurality of parallel adjacent channels each having an open top side, and at least one common supply line, into which one end of the adjacent channels open, wherein a portion of each of the adjacent channels forms a cylinder section of a cylinder/piston arrangement, with the other end of the adjacent channels forming an aperture sized to receive a piston;

a plurality of elongate pistons each associated with one of the plurality of channels and having a cross sectional area significantly less than the associated channel, wherein the pistons each have a free end which extends through the aperture into the cylinder section of one of the channels;

a cover cooperating with the top side of the substrate and configured to sealingly isolate the parallel adjacent channels from the outside environment; and

a sealing arrangement, which is stationary relative to the substrate, for sealing each of the pistons in an associated cylinder adjacent a second end of the channel;

wherein the pistons are configured to move axially through the sealing arrangement, enabling the free end to move into or out of the cylinder section; and

wherein each of the adjacent channels further comprise an open top functional channel section located between the cylinder channel section and the supply line, wherein the functional channel section has a significantly smaller cross sectional area than the cylinder channel section.

19. The microfluidic arrangement as claimed in claim 14, wherein the sealing arrangement comprises a continuous seal element which spans the plurality of adjacent channels and seals a plurality of pistons.

20. The microfluidic arrangement as claimed in claim 14, wherein the pistons each have a round cylindrical wire-like shape sized to freely move within the channel section without touching the cylinder side walls.